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Antifungal activity of endophytic Bacillus safensis B21 and its potential application as a biopesticide to control rice blast
Pesticide Biochemistry and Physiology ( IF 4.7 ) Pub Date : 2020-01-01 , DOI: 10.1016/j.pestbp.2019.09.003
Songhao Rong 1 , Hong Xu 1 , Lihua Li 1 , Rongjun Chen 1 , Xiaoling Gao 1 , Zhengjun Xu 1
Affiliation  

Endophytic bacteria are potential biocontrol agents for the control of fungal diseases. Here, an endophyte strain, B21, was isolated from Osmanthus fragrans Lour. fruits and identified as Bacillus safensis by analysis of its 16S rDNA gene sequence and its biochemical and physiological characteristics. The culture filtrate showed antifungal activity against Magnaporthe oryzae, which causes rice blast disease, and the IC50 of the methanol extract was 15.56 μg/mL, which was significantly lower than that of carbendazim (25.16 μg/mL). The antifungal activity of the methanol extract was stable at a wide range of pH values (1-9) and temperatures (40-100 °C). Two antifungal compounds were isolated by organic extraction, silica gel column chromatography and high-performance liquid chromatography (HPLC). Based on electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance spectrometry (NMR) analyses, the structures of the antifungal compounds were identified as iturin A2 and iturin A6. Additionally, the hyphae treated with iturin (iturin A2 or iturin A6) could be stained with the fluorescent dye propidium iodide (PI), indicating that these two compounds inhibited the growth of hyphae by changing the hyphal membrane permeability. In field experiments, spray treatment with fermentation broth resulted in a lower disease index than treatment with carbendazim, as did the culture filtrate. The results suggest that strain B21 and its bioactive compounds have the potential to be developed into a biopesticide for the biocontrol of rice blast.

中文翻译:

内生安全芽孢杆菌 B21 的抗真菌活性及其作为生物农药防治稻瘟病的潜在应用

内生细菌是控制真菌疾病的潜在生物防治剂。在这里,从 Osmanthus fragrans Lour 中分离出一种内生菌菌株 B21。通过分析其16S rDNA基因序列及其生化和生理特征,鉴定为安全芽孢杆菌。培养滤液对引起稻瘟病的稻瘟病菌具有抗真菌活性,甲醇提取物的IC50为15.56 μg/mL,明显低于多菌灵(25.16 μg/mL)。甲醇提取物的抗真菌活性在很宽的 pH 值 (1-9) 和温度 (40-100 °C) 范围内是稳定的。通过有机萃取、硅胶柱色谱和高效液相色谱 (HPLC) 分离出两种抗真菌化合物。基于电喷雾电离质谱 (ESI-MS) 和核磁共振光谱 (NMR) 分析,抗真菌化合物的结构被鉴定为 iturin A2 和 iturin A6。此外,用伊图林(iturin A2 或 iturin A6)处理的菌丝可以被荧光染料碘化丙啶 (PI) 染色,表明这两种化合物通过改变菌丝膜通透性来抑制菌丝的生长。在田间试验中,发酵液喷雾处理导致的疾病指数低于多菌灵处理,培养物滤液也是如此。结果表明,菌株B21及其生物活性化合物具有开发成为稻瘟病生物防治的生物农药的潜力。抗真菌化合物的结构被鉴定为iturin A2和iturin A6。此外,用伊图林(iturin A2 或 iturin A6)处理的菌丝可以被荧光染料碘化丙啶 (PI) 染色,表明这两种化合物通过改变菌丝膜通透性来抑制菌丝的生长。在田间试验中,发酵液喷雾处理导致的疾病指数低于多菌灵处理,培养物滤液也是如此。结果表明,菌株B21及其生物活性化合物具有开发成为稻瘟病生物防治的生物农药的潜力。抗真菌化合物的结构被鉴定为iturin A2和iturin A6。此外,用伊图林(iturin A2 或 iturin A6)处理的菌丝可以被荧光染料碘化丙啶 (PI) 染色,表明这两种化合物通过改变菌丝膜通透性来抑制菌丝的生长。在田间试验中,发酵液喷雾处理导致的疾病指数低于多菌灵处理,培养物滤液也是如此。结果表明,菌株B21及其生物活性化合物具有开发成为稻瘟病生物防治的生物农药的潜力。表明这两种化合物通过改变菌丝膜通透性来抑制菌丝的生长。在田间试验中,发酵液喷雾处理导致的疾病指数低于多菌灵处理,培养物滤液也是如此。结果表明,菌株B21及其生物活性化合物具有开发成为稻瘟病生物防治的生物农药的潜力。表明这两种化合物通过改变菌丝膜通透性来抑制菌丝的生长。在田间试验中,发酵液喷雾处理导致的疾病指数低于多菌灵处理,培养物滤液也是如此。结果表明,菌株B21及其生物活性化合物具有开发成为稻瘟病生物防治的生物农药的潜力。
更新日期:2020-01-01
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