Abstract It is well known that selenium (Se) is an essential element for humans, presenting antioxidant functions. This study aimed to evaluate the efficiency of Raphanus sativus L. as an agricultural crop indicated for selenium biofortification and to investigate the influence of different sources and forms of application on Se accumulation and macronutrient absorption. The experiment followed a randomized complete design with five replicates, arranged in a 2 × 2 + 1 factorial scheme, consisting of two sources of Se (sodium selenate and sodium selenite) and two forms of application (application in the soil at the dose of 1.2 mg kg−1 of Se and foliar application at the dose of 50 μmol L−1 of Se), with a control treatment without the addition of selenium. The accumulations of Se in the leaf, root, and whole plants were higher with the selenate source applied via soil when compared with the foliar application. For the selenite source applied via soil, Se root contents were higher when compared with the fertilization via leaf. The Se content in the root was lower for the selenate applied via soil when compared with the other treatments and the control. Raphanus sativus L is an agricultural crop suitable for the agronomic biofortification of selenium, since it concentrates the appropriate levels of Se in the roots and leaves, not harming the crop yield. The application of Se in the form of selenate and via soil was the best technique to increase the contents of Se in the plant, without causing damages in the accumulation of biomass, despite having led to a reduction in the accumulation of S.

中文翻译：

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含硒的生物强化作用及其对 Raphanus sativus L 中宏量营养素吸收的影响

摘要 众所周知，硒（Se）是人体必需的元素，具有抗氧化功能。本研究旨在评估萝卜作为用于硒生物强化的农作物的效率，并研究不同来源和施用形式对硒积累和常量营养素吸收的影响。实验遵循随机完整设计，有 5 个重复，以 2 × 2 + 1 因子方案排列，由两种硒来源（硒酸钠和亚硒酸钠）和两种施用形式（以 1.2 mg kg-1 硒和叶面喷施 50 μmol L-1 硒），对照处理不添加硒。硒在叶、根、与叶面施用相比，通过土壤施用硒酸盐来源的整株植物更高。对于通过土壤施用的亚硒酸盐源，与通过叶施肥相比，Se根含量更高。与其他处理和对照相比，通过土壤施用的硒酸盐根中的硒含量较低。Raphanus sativus L 是一种适用于硒的农艺生物强化的农作物，因为它在根和叶中浓缩了适当水平的硒，不会损害作物产量。以硒酸盐的形式和通过土壤施用硒是增加植物中硒含量的最佳技术，尽管导致了硫积累的减少，但不会对生物量的积累造成损害。