The aim of this study was to investigate antibacterial activity of Origanum compactum essential oils collected at three phenological stages on Escherichia coli and Bacillus subtilis. The antibacterial activity was evaluated using the agar-well diffusion assay. The MIC and MBC values were determined using the micro-dilution assay. The investigation of the antibacterial action was carried out by the evaluation of the effect of O. compactum essential oils on the antibacterial kinetic growth, the integrity of cell membrane and permeability of the cell membrane. The anti-quorum sensing activity was tested by the inhibition of the biofilm formation. The findings of this study showed that O. compactum essential oil has potent antibacterial activities against E. coli and B. subtilis. The lowest inhibition value against B. subtilis was obtained with O. compactum essential oil at the post-flowering stage (MIC = MBC = 0.0312% (v/v)). The antibacterial mechanisms of O. compactum essential oils are related to the disturbing of the cell membrane integrity and the increasing of the membrane permeability, which leads to the leakage of genetic materials (DNA and RNA). Moreover, O. compactum essential oils inhibited the formation of the biofilms, a phenotype that has been known to be quorum sensing regulated.

这项研究的目的是研究在三个物候阶段收集的牛至精油对大肠杆菌和枯草芽孢杆菌的抗菌活性。使用琼脂孔扩散测定法评估抗菌活性。使用微量稀释测定法测定MIC和MBC值。抗菌作用的研究是通过评价紧密密穗麦草精油对抗菌动力学生长，细胞膜完整性和细胞膜通透性的影响来进行的。通过抑制生物膜形成来测试抗群体感应活性。这项研究的结果表明，致密O. compactum精油对大肠杆菌和枯草芽孢杆菌具有有效的抗菌活性。在开花后阶段，用紧凑型芽孢杆菌精油获得对枯草芽孢杆菌的最低抑制值（MIC = MBC = 0.0312％（v / v））。致密欧根草精油的抗菌机制与细胞膜完整性的破坏和膜通透性的增加有关，这导致遗传物质（DNA和RNA）的泄漏。此外，紧凑型香茅（O.compactum）精油抑制了生物膜的形成，该生物膜是已知的群体感应调节的表型。