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Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS
Journal of Pharmaceutical Analysis ( IF 8.8 ) Pub Date : 2019-11-27 , DOI: 10.1016/j.jpha.2019.11.007
Ting Liu , Ruiyun Li , Yue Cui , Zhiguo Yu , Yunli Zhao

A metabonomic approach involving an ultrahigh-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) was used to investigate the changes in the endogenous metabolites in the plasma of rats with yeast-induced pyrexia treated with Gegenqinlian decoction (GQLD), aspirin and itraconazole. The differences in the small molecule profiles of treatment using traditional Chinese medicine, etiological treatment and symptomatic treatment were elucidated. Thirty-six plasma metabolites were identified or putatively identified, and the effects of the three medicines on the thirty-six metabolites were studied. Their metabolic pathways indicated that GQLD, aspirin and itraconazole ameliorated the rats with yeast-induced pyrexia predominantly by regulating the metabolisms of phospholipid, sphingolipid, fatty acid oxidation, fatty acid amides, amino acid and glycerolipid in vivo. The pharmacodynamics and metabonomic results showed that the three medicines exhibited the therapeutic effects on pyrexia by regulating the perturbations of multiple metabolisms. The study provided a scientific basis for an in-depth understanding of the therapeutic effects of GQLD, aspirin and itraconazole on rats with yeast-induced pyrexia.



中文翻译:

葛根勤联汤,阿司匹林和伊曲康唑对发热大鼠血浆生化变化的代谢组学分析

代谢组学方法涉及超高效液相色谱与傅里叶变换离子回旋共振质谱(UHPLC-FT-ICR-MS)的结合,用于研究酵母致发热的大鼠血浆中内源性代谢产物的变化,葛根勤联汤,阿司匹林和伊曲康唑。阐明了中药治疗,病因治疗和对症治疗中小分子谱的差异。鉴定或假定鉴定了36种血浆代谢物,并研究了这三种药物对36种代谢物的作用。它们的代谢途径表明,GQLD,阿司匹林和伊曲康唑主要通过调节磷脂的代谢来改善酵母引起的发热的大鼠,药效学和代谢组学结果表明,这三种药物通过调节多种新陈代谢的扰动,对发热有治疗作用。该研究为深入了解GQLD,阿司匹林和伊曲康唑对酵母致发热的大鼠的治疗作用提供了科学依据。

更新日期:2019-11-27
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