BackgroundThe aim was to examine the impact of lipopolysaccharide-induced systemic inflammation on expression of mRNA for cocaine- and amphetamine-regulated transcript (CART) and the thyrotropin receptor (TSHR) and its ligands in CNS areas of relevance for feeding controls and metabolism. Lipopolysaccharide effects on plasma levels of TSH and CART peptides were also examined.MethodsLipopolysaccharide (150–200 μg/mouse) was injected in C57BL/6J mice and tissue and plasma samples taken after 24 h. To establish if plasma increase in CART peptide levels were prostanoid dependent, indomethacin was given via the drinking water beginning 48 h prior to LPS. We evaluated mRNA expression for CART, TSHR, TSHβ, and thyrostimulin in brain and pituitary extracts. Plasma levels of TSH, CARTp, and serum amyloid P component were analyzed by ELISA.ResultsLipopolysaccharide suppressed TSHR mRNA expression in the arcuate nucleus and the pituitary. CART mRNA expression was reduced in the arcuate nucleus but elevated in the pituitary of mice treated with Lipopolysaccharide, whereas plasma TSH remained unchanged. Plasma CART peptide concentration increased after LPS treatment in a prostanoid-independent manner, and CART peptide levels correlated positively to degree of inflammation.ConclusionsOur findings suggest that central and peripheral CART is affected by acute inflammation. Considering the role of the arcuate nucleus in feeding controls, our data highlight TSHR and CART as putative neuroendocrine signaling components that respond to inflammation, perhaps to maintain weight and metabolic homeostasis during states of disease.

中文翻译：

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LPS 免疫攻击减少弓状核 TSHR 和 CART mRNA 并提高血浆 CART 肽

背景目的是检查脂多糖诱导的全身炎症对可卡因和苯丙胺调节转录物 (CART) 和促甲状腺激素受体 (TSHR) 及其配体在与喂养控制和代谢相关的 CNS 区域的 mRNA 表达的影响。还检查了脂多糖对 TSH 和 CART 肽血浆水平的影响。方法将脂多糖（150-200 μg/小鼠）注射到 C57BL/6J 小鼠体内，并在 24 小时后采集组织和血浆样本。为了确定 CART 肽水平的血浆增加是否依赖于前列腺素，在 LPS 前 48 小时开始通过饮用水给予吲哚美辛。我们评估了脑和垂体提取物中 CART、TSHR、TSHβ 和甲状腺刺激素的 mRNA 表达。通过ELISA分析TSH、CARTp和血清淀粉样蛋白P成分的血浆水平。结果脂多糖抑制弓状核和垂体中TSHR mRNA的表达。CART mRNA 在弓状核中的表达降低，但在用脂多糖处理的小鼠的垂体中升高，而血浆 TSH 保持不变。LPS 治疗后血浆 CART 肽浓度以前列腺素非依赖性方式增加，CART 肽水平与炎症程度呈正相关。结论我们的研究结果表明，中枢和外周 CART 受急性炎症的影响。考虑到弓状核在喂养控制中的作用，我们的数据突出显示 TSHR 和 CART 作为对炎症作出反应的推定神经内分泌信号成分，可能是为了在疾病状态下维持体重和代谢稳态。