Trachyspermum ammi (L.) Sprague, commonly known as ‘Ajowan’, belongs to the family ‘Apiaceae’. Ajowan fruits yield 2–6% essential oil, and thymol is the major constituent of the oil (35–60%). T. ammi is of high medicinal value; however, the genomic resources for this medicinal plant are rare. To obtain transcript sequences of ajowan inflorescence, RNA-Seq was applied using Illumina HiSeq 2000 platform. Following de novo assembly, 68051 unigenes were produced, among which 43156 unigenes were annotated against different sequence databases. Blastx results of ajowan unigenes showed that 41950 and 29273 unigenes were hit in the NCBI non-redundant (Nr) protein and UniProt databases, respectively. Gene Ontology (GO) classification showed that 29258 unigenes were categorized into 55 GO terms, and 26002 unigenes were clustered in the Orthologous Group categories. Blastx results against the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database indicated that 14986 unigenes were represented in 127 pathways. In addition, 8751 sequences containing simple sequence repeats (SSRs) and 1180 putative transcription factor genes were also identified. As far as we know, this repository of genomic information is the first resource currently available for transcriptome characterization, gene discovery, developing SSR molecular markers and future genomic research on ajowan.

中文翻译：

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Ajowan（Tramchyspermum ammi L.）花序的转录组分析

Trachyspermum ammi（L.）Sprague，俗称“ Ajowan”，隶属于“ Apiaceae”家族。阿霍旺（Ajowan）果实可产生2–6％的精油，百里香酚是该油的主要成分（35–60％）。T.阿米具有很高的药用价值；但是，这种药用植物的基因组资源很少。为了获得阿霍万花序的转录本序列，使用Illumina HiSeq 2000平台应用RNA-Seq。从头组装后，产生了68051个单基因，其中43156个单基因针对不同的序列数据库进行了注释。Ajowan单基因的Blastx结果表明，在NCBI非冗余（Nr）蛋白和UniProt数据库中分别击中了41950和29273个单基因。基因本体论（GO）分类显示，将29258个单基因分类为55个GO术语，并将26002个单基因聚类在直系同源群类别中。Blastx针对京都议定书基因与基因组百科全书（KEGG）途径数据库的结果表明，在127条途径中代表了14986个单基因。此外，还鉴定了8751个包含简单序列重复（SSR）的序列和1180个推定的转录因子基因。据我们所知，这个基因组信息库是当前可用于转录组表征，基因发现，开发SSR分子标记以及未来有关ajowan的基因组研究的第一个资源。