Butea monosperma is a medicinal plant extensively used in ayurveda and is known for its medicinal applications. Butea monosperma seed powder is used for treating various health ailments including cancer. Although, most parts of the plant are being used in medicine none of them except for seeds are known to contain lectins. A lectin from seeds has been reported earlier, however it was not tested for its anticancer properties. Since plant lectins are known for their anticancer properties, in order to explore anticancer potential of B. monosperma a legume lectin was purified from seeds in a single step by nine fold, by a modified method using affinity chromatography on lactose-Sepharose 4B column and tested for its anticancer properties against human hepatocellular carcinoma cells. SDS PAGE of BML reveals two bands with Mr of 32 and 34 kDa and a minor band of 67 kDa similar to earlier reported BML. Hapten inhibition assay shows that lectin is specific to GalNAc, galactose, lactose and D-Fucose. BML shows strong binding to Hep G2 cells with MFI of 476. MTT assay showed growth inhibitory effect on Hep G2 cells by BML, PTL1 and PTL2 in a dose and time dependent manner. Growth inhibitory effect of BML on Hep G2 cells was compared with two legume lectins, PTL1 and PTL2 from Psophocarpus tetragonolobus of Fabaceae Family. BML, PTL1 and PTL2 showed growth inhibitory effect on Hep G2 cells. The presence of lectin, in B. monosperma seeds contribute for its anticancer properties.

中文翻译：

---

一种从精子种子中纯化N-乙酰半乳糖胺特异性凝集素的改进方法及其对人肝癌细胞生长的影响

单子叶丁香是广泛用于阿育吠陀的药用植物，并因其药用用途而闻名。紫丁香种子粉可用于治疗各种健康疾病，包括癌症。尽管该植物的大部分部位都被用于医学，但除了种子外，没有其他植物中含有凝集素。种子中的凝集素已经有较早的报道，但是尚未测试其抗癌特性。由于植物凝集素因其抗癌特性而闻名，为了探索单子芽孢杆菌的抗癌潜力通过改良方法在乳糖-琼脂糖4B柱上使用亲和色谱法从种子中纯化豆科植物凝集素9倍，并测试其对人肝癌细胞的抗癌特性。BML的SDS PAGE揭示了两条带，其Mr分别为32和34 kDa，一条较小的带为67 kDa，与先前报道的BML相似。半抗原抑制测定表明，凝集素对GalNAc，半乳糖，乳糖和D-岩藻糖具有特异性。BML显示与Hep G2细胞的强结合​​，MFI为476。MTT分析显示BML，PTL1和PTL2以剂量和时间依赖性方式抑制Hep G2细胞的生长。将BML对Hep G2细胞的生长抑制作用与两种豆类植物凝乳素（Psophocarpus tetragonolobus）的PTL1和PTL2进行了比较。法科家族的成员。BML，PTL1和PTL2对Hep G2细胞具有生长抑制作用。B. monosperma种子中存在的凝集素为其抗癌特性做出了贡献。