The Cullin-RING E3 ubiquitin ligase (CRL) complex is the most common E3 ligase, and the SCF complex (CRL1) has the most diverse functions. Cullin1(CUL1) is a scaffolding protein for assembly of the complex. SCF has been shown to participate in the non-biological stress response pathways. In this study, a classic CUL1 protein was identified in Solanum tuberosum, StCUL1. A full-length cDNA of the StCUL1 gene was obtained from ED13 (a potato variety) by Ralstonia solanacearum inoculation using the RACE method. Sequence analysis indicated that the gene comprised 2662 bp, with an open reading frame of 2229 bp encoding 743 amino acids. The expression levels of the StCUL1 gene in potato treated with R. solanacearum and exogenous hormones (such as salicylic acid, jasmonic acid methyl ester and abscisic acid) at different time points were determined by real-time PCR. The results indicated that StCUL1 was induced not only by pathogenic bacteria, but also by exogenous hormones, with sustained high expression. However, there were some differences in the modes of expression. Tissue localization analysis indicated that its expression was tissue specific, and it was mainly in the phloem of the vascular system of stems and leaves.

中文翻译：

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马铃薯StCUL1基因的克隆与表达分析

Cullin-RING E3泛素连接酶（CRL）复合物是最常见的E3连接酶，而SCF复合物（CRL1）具有最多样化的功能。Cullin1（CUL1）是用于组装复合物的支架蛋白。已经证明SCF参与非生物应激反应途径。在这项研究中，一个经典CUL1蛋白被认定马铃薯，StCUL1。通过使用RACE法接种青枯雷尔氏菌，从ED13（马铃薯品种）获得StCUL1基因的全长cDNA 。序列分析表明该基因占2662 bp，开放阅读框2229 bp，编码743个氨基酸。马铃薯处理后StCUL1基因的表达水平。通过实时荧光定量PCR测定青枯菌和外源激素（例如水杨酸，茉莉酸甲酯和脱落酸）在不同的时间点。结果表明，StCUL1不仅被致病菌诱导，还被外源激素诱导，并持续高表达。但是，表达方式存在一些差异。组织定位分析表明其表达是组织特异性的，并且主要在茎和叶的血管系统的韧皮部中表达。