In this study, genetic diversity of agro-morphological traits and population structure of 167 chickpea were assessed using genomic-SSR and EST-SSR molecular markers. Field evaluation of chickpea genotypes showed highly variation for most of measured characteristics such as: number of pods per plant, number of seeds per plant, 100-seed weight and seed yield. Cluster analysis based on morphological traits, grouped chickpea genotypes into four clusters. 37 SSR markers showed highly allelic variation across chickpea genotypes with an average value of 2.95 alleles per locus. Polymorphism information content of SSR locus ranged from 0.27 to 0.80, with an average value of 0.50. SSR-based cluster analysis, grouped 167 chickpea genotypes into two clusters, while cluster I and II divided into five and four sub-clusters. Population structure and discriminant analysis of principal components showed to presence of two clusters within the chickpea, while there was no strong correlation between morphological-based clustering and molecular genetic diversity. Our results showed highly genetic diversity for both agro-morphological and molecular analysis in examined chickpea genotypes. Overall, results of present study can be used for germplasm management and particularly for introducing new genotypes with desirable characteristics for using in breeding programs.

中文翻译：

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基因组-SSR和ESR-SSR分子标记对鹰嘴豆的农业形态描述，遗传多样性和种群结构

本研究利用基因组SSR和EST-SSR分子标记对167种鹰嘴豆的农艺性状遗传多样性和种群结构进行了评估。鹰嘴豆基因型的田间评估显示，大多数测量特性存在很大差异，例如：每株豆荚的数量，每株种子的数量，100粒重和种子产量。根据形态特征进行聚类分析，将鹰嘴豆基因型分为四个聚类。37个SSR标记在鹰嘴豆基因型之间显示高度等位基因变异，每个基因座平均2.95个等位基因。SSR基因座的多态信息含量范围为0.27至0.80，平均值为0.50。基于SSR的聚类分析将167种鹰嘴豆基因型分为两个聚类，而聚类I和II分为五个和四个亚类。种群结构和主要成分的判别分析表明，鹰嘴豆中存在两个簇，而基于形态的簇与分子遗传多样性之间没有强相关性。我们的结果显示，在所检查的鹰嘴豆基因型中，农业形态和分子分析均具有高度的遗传多样性。总的来说，本研究的结果可用于种质管理，尤其是可用于引入具有理想特性的新基因型以用于育种程序。我们的结果显示，在所检查的鹰嘴豆基因型中，农业形态和分子分析均具有高度的遗传多样性。总体而言，本研究的结果可用于种质管理，尤其是可用于引入具有理想特性的新基因型以用于育种程序。我们的结果显示，在所检查的鹰嘴豆基因型中，农业形态和分子分析均具有高度的遗传多样性。总体而言，本研究的结果可用于种质管理，尤其是可用于引入具有理想特性的新基因型以用于育种程序。