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Molecular detection and in silico characterization of cold shock protein coding gene ( csp A) from cold adaptive Pseudomonas koreensis
Journal of Plant Biochemistry and Biotechnology ( IF 1.9 ) Pub Date : 2019-03-27 , DOI: 10.1007/s13562-019-00500-8 Srikant Awasthi , Anjney Sharma , Pragya Saxena , Jagriti Yadav , K. Pandiyan , M. Kumar , Arjun Singh , Hillol Chakdar , Arpan Bhowmik , Prem L. Kashyap , Alok K. Srivastava , Anil K. Saxena
Journal of Plant Biochemistry and Biotechnology ( IF 1.9 ) Pub Date : 2019-03-27 , DOI: 10.1007/s13562-019-00500-8 Srikant Awasthi , Anjney Sharma , Pragya Saxena , Jagriti Yadav , K. Pandiyan , M. Kumar , Arjun Singh , Hillol Chakdar , Arpan Bhowmik , Prem L. Kashyap , Alok K. Srivastava , Anil K. Saxena
Cold shock proteins (CSPs) are greatly conserved family of structurally related DNA binding proteins which are produced during temperature drift. A 213 bp long cspA gene was cloned and sequenced from Pseudomonas koreensis P2 in the present study. The expression analysis of the cspA showed > 2.5 folds increase in the mRNA level at 15 °C while the expression was almost on par at 30 °C and 5 °C indicating its role in moderately low temperature. In silico analyses of the gene showed that the gene codes for 7.69 kDa protein which was phylogenetically very similar to CspA present in Pseudomonads. Amino acid composition of the CspA from P. koreensis was different from that of mesophilic Pseudomonas and tiny/small amino varied significantly between CspA of cold adaptive and mesophilic species. The CspA from P. koreensis P2 contained RNP motifs involved in binding of DNA and RNA. Phylogenetic analyses revealed that the CspA protein of P. koreensis P2 was more close to CspA of distant subgroups of Pseudomonas like P. fluorescens and P. putida subgroup indicating a possible intra-specific gene transfer.
中文翻译:
朝鲜冷适应性假单胞菌冷休克蛋白编码基因(csp A)的分子检测和计算机表征
冷激蛋白(CSP)是在温度漂移过程中产生的结构相关的DNA结合蛋白的高度保守的家族。在本研究中,从韩国假单胞菌P2克隆并测序了一个213 bp长的csp A基因。csp A的表达分析显示,在15°C时,mRNA水平增加> 2.5倍,而在30°C和5°C时,表达水平几乎相等,表明其在中等低温下的作用。在计算机上对该基因进行的计算机分析表明,该基因编码7.69 kDa蛋白,在系统发育上与假单胞菌中存在的CspA非常相似。所述的CspA从氨基酸组成P. koreensis是从嗜温的不同假单胞菌冷适应性物种和嗜温性物种的CspA之间的微小/小氨基差异显着。来自韩国假单胞菌P2的CspA含有参与DNA和RNA结合的RNP基序。系统发育分析表明,朝鲜假单胞菌P2的CspA蛋白更接近于假单胞菌的遥远亚群,如荧光假单胞菌和恶臭假单胞菌亚群的CspA,表明可能存在种内基因转移。
更新日期:2019-03-27
中文翻译:
朝鲜冷适应性假单胞菌冷休克蛋白编码基因(csp A)的分子检测和计算机表征
冷激蛋白(CSP)是在温度漂移过程中产生的结构相关的DNA结合蛋白的高度保守的家族。在本研究中,从韩国假单胞菌P2克隆并测序了一个213 bp长的csp A基因。csp A的表达分析显示,在15°C时,mRNA水平增加> 2.5倍,而在30°C和5°C时,表达水平几乎相等,表明其在中等低温下的作用。在计算机上对该基因进行的计算机分析表明,该基因编码7.69 kDa蛋白,在系统发育上与假单胞菌中存在的CspA非常相似。所述的CspA从氨基酸组成P. koreensis是从嗜温的不同假单胞菌冷适应性物种和嗜温性物种的CspA之间的微小/小氨基差异显着。来自韩国假单胞菌P2的CspA含有参与DNA和RNA结合的RNP基序。系统发育分析表明,朝鲜假单胞菌P2的CspA蛋白更接近于假单胞菌的遥远亚群,如荧光假单胞菌和恶臭假单胞菌亚群的CspA,表明可能存在种内基因转移。