In this study, it was aimed to develop drug eluting biodegradable and biocompatible bone supportive fibers and films, which can locally be applicable on the infected bone defect area for osteomyelitis treatment. For this purpose, vancomycin (Vm) loaded gelatin (G) microspheres were prepared and dispersed into poly(ε-caprolactone) (PCL) solution and then the suspension was processed as films and fibers by solvent casting and wet spinning techniques, respectively. The mean particle size distribution and morphology of Vm loaded G microspheres were characterized by laser diffraction method and scanning electron microscopy, respectively. In vitro Vm release profiles and release kinetics from microsphere, fiber and film formulations were investigated. In vitro biodegradation properties of fiber and film formulations were examined in both hydrolytic and enzymatic media during 25 days period. The cytotoxicity of Vm eluting films and fibers were tested on L929 cells by MTT assay. Presence of PCL in film and fiber formulations retarded the release of Vm from microspheres and provided long term sustained release. Vm eluting films and fibers exhibited strong antibacterial properties against Staphylococcus aureus and Staphylococcus epidermidis.

在这项研究中，其目的是开发可生物降解和生物相容性的骨支持纤维和薄膜的药物洗脱方法，该方法可局部应用于感染的骨缺损区域，以治疗骨髓炎。为此，制备了载有万古霉素（Vm）的明胶（G）微球，并将其分散在聚（ε-己内酯）（PCL）溶液中，然后分别通过溶剂浇铸和湿法纺丝将悬浮液加工成薄膜和纤维。用激光衍射法和扫描电镜分别表征了负载Vm的G微球的平均粒径分布和形貌。研究了微球，纤维和薄膜制剂的体外Vm释放曲线和释放动力学。体外在25天的时间内，在水解和酶促介质中检查了纤维和薄膜配方的生物降解特性。通过MTT法在L929细胞上测试了Vm洗脱膜和纤维的细胞毒性。薄膜和纤维配方中PCL的存在会延迟Vm从微球的释放，并提供长期持续释放。Vm洗脱膜和纤维对金黄色葡萄球菌和表皮葡萄球菌表现出较强的抗菌性能。