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Stability Enhancement of Silver Nanoparticles Through Surface Encapsulation via a Facile Green Synthesis Approach and Toxicity Reduction
Journal of Inorganic and Organometallic Polymers and Materials ( IF 4 ) Pub Date : 2019-11-04 , DOI: 10.1007/s10904-019-01373-z
Mahmuda Akter , A. K. M. Atique Ullah , Md. Shiblur Rahaman , Md. Mostafizur Rahman , Md. Tajuddin Sikder , Toshiyuki Hosokawa , Takeshi Saito , Masaaki Kurasaki

Persuaded by the necessity of reduction of toxicity, silver nanoparticles (Ag-NPs) were synthesized from the reaction between AgNO3 solution and Mizuna (Brassica rapa var. japonica) leaf extract. This study aimed to investigate the role of synthesis temperature on particle properties. Thus, four synthesis-temperatures, 25 °C (room temperature), 60 °C, 80 °C, and 100 °C were applied. Ag-NPs were characterized using ultraviolet–visible (UV–Vis) spectrophotometer, energy dispersive X-ray spectrometer, X-ray diffractometer, transmission electron microscope, atomic absorption spectrometry and dynamic light scattering techniques. The high-temperature (80 and 100 °C) synthesis showed higher stability, demostrated lowest release of silver ion (0.02 µg/mL, 0.002 µg/mL) compared to low-temperature synthesis. In addition, high-temperature synthesis showed higher C-content (> 50%) with lower Ag-content (< 30%) than low-temperature (25 and 60 °C) synthesis. Moreover, a complete surface encapsulation is found on the particles synthesised at 100 °C by TEM analysis. The synthesized Ag-NPs were exposed to Caco-2 cell lines for cytotoxicity assessment. The high-temperature synthesis Ag-NPs exerted significantly lower cytotoxicity in terms of Caco-2 cell viability and lactate dehydrogenase (LDH) activity assay. Upon exposure to 1, 5 and 10 µg/mL Ag-NPs for 48 h the cell viability (96.33%, 90.66% and 89.66%) was not changed for high-temperature synthesis particles as well as the LDH activity was unchanged. However, low-temperature synthesis showed significant cytotoxicity (81%, 66% and 44.33%) under similar experimental conditions. Thus, characterization and cytotoxicity techniques clearly demonstrate the successful synthesis of Ag-NPs with the encapsulation of bio-molecules that enhances the particle stability and reduce cytotoxicity.

中文翻译:

通过一种简便的绿色合成方法通过表面包封和降低毒性来增强银纳米颗粒的稳定性

通过还原毒性的必要性说服,银纳米颗粒(银纳米颗粒)从之间的AgNO反应,合成3溶液和水菜(芜菁变种。粳稻)叶提取物。这项研究旨在研究合成温度对颗粒性质的影响。因此,施加了25℃(室温),60℃,80℃和100℃的四个合成温度。使用紫外可见分光光度计,能量色散X射线光谱仪,X射线衍射仪,透射电子显微镜,原子吸收光谱法和动态光散射技术对Ag-NPs进行了表征。高温合成(80和100°C)显示出更高的稳定性,与低温合成相比,银离子的释放最低(0.02 µg / mL,0.002 µg / mL)。此外,与低温(25和60°C)合成相比,高温合成显示更高的C含量(> 50%)和更低的Ag含量(<30%)。而且,通过TEM分析,发现在100°C合成的颗粒上有完整的表面包封。将合成的Ag-NPs暴露于Caco-2细胞系以进行细胞毒性评估。就Caco-2细胞活力和乳酸脱氢酶(LDH)活性测定而言,高温合成Ag-NPs的细胞毒性显着降低。暴露于1、5和10 µg / mL的Ag-NP 48小时后,高温合成颗粒的细胞活力(96.33%,90.66%和89.66%)不变,并且LDH活性不变。但是,在相似的实验条件下,低温合成显示出明显的细胞毒性(81%,66%和44.33%)。因此,
更新日期:2019-11-04
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