Synthesis of (3-aminopropyl) triethoxysilane (APTES)-functionalized graphene oxide (GO) nanosheets, statistical optimization of conditions for immobilization of Bacillus atrophaeus lipase (BaL) on as-synthesized support, and application of the immobilized BaL for esterification of valeric acid were carried out in this investigation. The optimum specific activity of the immobilized BaL (81.60 ± 0.28 U mg⁻¹) was achieved at 3 mg mL⁻¹ of GO-NH₂, 50 mM of phosphate buffer, pH 7.0, 60 min sonication time, 100 mM glutaraldehyde, 25 U mL⁻¹ of enzyme, and 8 h immobilization time at 4 °C. The immobilized BaL retained about 90% of its initial activity after 10 days of storage. Moreover, about 70% of the initial activity of the immobilized BaL was retained after 10 cycles of application. The results of esterification studies exhibited that maximum pentyl valerate synthesis using the free BaL (34.5%) and the immobilized BaL (92.7%) occurred in the organic solvent medium (xylene) after 48 h of incubation at 60 °C.

（3-氨基丙基）三乙氧基硅烷（APTES）-功能化的氧化石墨烯（GO）纳米片的合成，萎缩芽孢杆菌脂肪酶（Ba L）固定在合成载体上的条件的统计优化，以及固定化Ba L在酯化中的应用在该研究中进行了戊酸。固定化的最佳比活性的Ba L（81.60±0.28毫克ü ^-1）以3mg毫升达到^-1 GO-NH的₂，磷酸盐缓冲液50mM的，pH为7.0，60分钟超声处理时间，100mM的戊二醛， 25 U mL ^-1的酶，在4°C下固定8小时。固定BaL在储存10天后保留了其初始活性的90％。而且，在10个施用循环后，固定的Ba L的初始活性的约70％得以保留。酯化研究的结果表明，在60°C孵育48小时后，使用游离Ba L（34.5％）和固定化Ba L（92.7％）的最大戊酸戊酯合成发生在有机溶剂介质（二甲苯）中。