Creating scaffolds for skin tissue engineering remain challenging in terms of their mechanical and biological properties. In this paper, we present a study on the nanocomposite polyurethane (PU)/polycaprolactone (PCL) scaffolds with graphene oxide (GO), which were fabricated by using electrospinning method, for potential skin tissue engineering. For this, homogenous and soft PU nanofibers containing varying percent of polycaprolactone (12% and 15%) and nano GO (0.5–4%) were electrospun, respectively, and then characterized by different techniques/assays in vitro. For the scaffold characterization, scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) were used. The SEM results show the spun scaffolds have 3D porous structure (90%) with the fiber diameter increased with the GO concentration, while the FTIR results confirmed the presence of PU, PCL, and Go in the scaffolds. Also, the biocompatibility, via the cytotoxicity, of the scaffolds was examined by MTT assay with the human skin fibroblast cells, along with their wettability in terms of contact angle. Our results show that the scaffolds are biocompatible to the skin fibroblast cell, illustrating their potential use in skin tissue engineering. Also, our results illustrate that the addition of GO to the PU/PCL composite can increase the wettability (or hydrophilicity) and biocompatibility of scaffolds. Combined together, the nanocomposite PU/PCL scaffolds with GO are promising as biocompatible constructs for skin tissue engineering.

就皮肤组织工程而言，创建支架在机械和生物学特性方面仍然具有挑战性。在本文中，我们提出了通过电纺丝方法制备的纳米复合聚氨酯（PU）/聚己内酯（PCL）支架和氧化石墨烯（GO）的研究，用于潜在的皮肤组织工程。为此，分别电纺包含不同百分比的聚己内酯（12％和15％）和纳米GO（0.5–4％）的均质和柔软PU纳米纤维，然后在体外采用不同的技术/方法进行表征。对于支架的表征，使用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）。SEM结果表明，纺丝支架具有3D多孔结构（90％），且纤维直径随GO浓度的增加而增加，FTIR结果证实了支架中存在PU，PCL和Go。而且，通过MTT测定法与人皮肤成纤维细胞一起，通过细胞毒性对支架的生物相容性以及接触角方面的润湿性进行了检查。我们的结果表明，支架与皮肤成纤维细胞具有生物相容性，说明它们在皮肤组织工程中的潜在用途。同样，我们的结果表明，将GO添加到PU / PCL复合材料中可以增加支架的润湿性（或亲水性）和生物相容性。结合在一起，具有GO的纳米复合PU / PCL支架有望作为皮肤组织工程的生物相容性构建体。用人皮肤成纤维细胞通过MTT测定法检查支架的数量，以及它们在接触角方面的可湿性。我们的结果表明，支架与皮肤成纤维细胞具有生物相容性，说明它们在皮肤组织工程中的潜在用途。同样，我们的结果表明，将GO添加到PU / PCL复合材料中可以增加支架的润湿性（或亲水性）和生物相容性。结合在一起，具有GO的纳米复合PU / PCL支架有望作为皮肤组织工程的生物相容性构建体。用人皮肤成纤维细胞通过MTT测定法检查了支架的数量，以及它们在接触角方面的可湿性。我们的结果表明，支架与皮肤成纤维细胞具有生物相容性，说明它们在皮肤组织工程中的潜在用途。同样，我们的结果表明，将GO添加到PU / PCL复合材料中可以增加支架的润湿性（或亲水性）和生物相容性。结合在一起，具有GO的纳米复合PU / PCL支架有望作为皮肤组织工程的生物相容性构建体。我们的结果表明，将GO添加到PU / PCL复合材料中可以增加支架的润湿性（或亲水性）和生物相容性。结合在一起，具有GO的纳米复合PU / PCL支架有望作为皮肤组织工程的生物相容性构建体。我们的结果表明，将GO添加到PU / PCL复合材料中可以增加支架的润湿性（或亲水性）和生物相容性。结合在一起，具有GO的纳米复合PU / PCL支架有望作为皮肤组织工程的生物相容性构建体。