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Stretchable collagen-coated polyurethane-urea hydrogel seeded with bladder smooth muscle cells for urethral defect repair in a rabbit model.
Journal of Materials Science: Materials in Medicine ( IF 3.7 ) Pub Date : 2019-12-04 , DOI: 10.1007/s10856-019-6342-7
Chengyuan Wang 1 , Chunyang Chen 1 , Mingyu Guo 2 , Bin Li 2 , Fengxuan Han 2 , Weiguo Chen 1
Affiliation  

The major challenge to treat the clinical adverse effects of long-segment urethra is in achieving viable tissue substitution. The substituted construct's properties-such as its resilience, contraction, and ability to minimize scar-stenosis formation should be considered. In the present work, a unique polyurethane-urea (PUU) fibrous membrane is fabricated by electrospinning. Then PUU was coated by collagen and formed the elasticity hydrogel after immersed in collagen solution. Meanwhile, the cPUU hydrogel exhibited a fibrous microstructure. This cPUU hydrogel had outstanding stretching property with 404 ± 40% elongation at break compared with traditional hydrogels, which satisfied the requirement of urethra. The cPUU hydrogel also supported the adhesion and growth of bladder smooth-muscle cells (BSMCs) in natural state cell morphology. Urethral defects in New Zealand male rabbits were repaired with cPUU seeded with BSMCs in vivo. After three months, more smooth-surface area of reconstructed urethral tissues was observed in the cPUU hydrogel-BMSCs groups compared with that of the control group. The luminal patency and the incidence of complications-including calculus formation, urinary fistula, and urethral-stricture occurrence were significantly lower in the cPUU group compared with that of the control group. Hence, cPUU fibrous hydrogels are promising scaffolds for application in urological tissue engineering.

中文翻译:

可伸缩的胶原蛋白涂覆的聚氨酯-尿素水凝胶接种了膀胱平滑肌细胞,用于兔模型中的尿道缺损修复。

治疗长段尿道的临床不良反应的主要挑战是实现可行的组织替代。应考虑替代结构的特性,例如其回弹性,收缩力和最小化疤痕狭窄形成的能力。在目前的工作中,通过静电纺丝制备了独特的聚氨酯-尿素(PUU)纤维膜。然后将PUU涂上胶原蛋白,并浸入胶原蛋白溶液中,形成弹性水凝胶。同时,cPUU水凝胶表现出纤维微结构。与传统的水凝胶相比,该cPUU水凝胶具有出色的拉伸性能,断裂伸长率为404±40%,可以满足尿道的要求。cPUU水凝胶还支持膀胱平滑肌细胞(BSMC)在自然状态细胞形态下的粘附和生长。体内植入BSMC的cPUU修复了新西兰雄兔的尿道缺陷。三个月后,与对照组相比,cPUU水凝胶-BMSCs组的尿道组织重建表面更光滑。与对照组相比,cPUU组的管腔通畅性和并发症(包括牙结石形成,尿瘘和尿道狭窄发生率)的发生率显着降低。因此,cPUU纤维水凝胶是用于泌尿外科组织工程的有前途的支架。与对照组相比,cPUU组的管腔通畅性和并发症(包括牙结石形成,尿瘘和尿道狭窄发生率)的发生率显着降低。因此,cPUU纤维水凝胶是用于泌尿外科组织工程的有前途的支架。与对照组相比,cPUU组的管腔通畅性和并发症(包括牙结石形成,尿瘘和尿道狭窄发生率)的发生率显着降低。因此,cPUU纤维水凝胶是用于泌尿外科组织工程的有前途的支架。
更新日期:2019-12-04
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