The aim of this research was to formulate antimicrobial, composite films of guar gum, sago starch, and whey protein isolate for the prophylaxis of the bacterial gastroenteritis. The model antibacterial agents incorporated were essential oils, namely, carvacrol, citral and their combination. The films became darker and brownish in color due to the entrapment of the oils. The surface of the oil-entrapped films was more rough and coarse compared to the control film. Confocal micrographs affirmed the uniform distribution of the oil droplets within the biopolymeric network. The highest crystallite size and lowest lattice strain were estimated in the citral-containing film. FTIR analysis demonstrated that the incorporation of citral increased the proportion of the β-sheet structures of the whey protein isolate within the film matrix. However, the film formulation containing combination of carvacrol and citral demonstrated the lowest water vapor transmission rate (WVTR), highest tensile strength, Young’s modulus and work to failure. All the oil-containing films demonstrated good antibacterial potency against the model bacterial gastroenteritis causing bacteria, namely, Bacillus cereus and Escherichia coli. In gist, it can be concluded that the prepared antimicrobial films could be used for the prophylaxis of the bacterial gastroenteritis.

这项研究的目的是配制抗菌，瓜耳胶，西米淀粉和乳清蛋白分离物的复合膜，以预防细菌性肠胃炎。加入的典型抗菌剂是香精油，即香芹酚，柠檬醛及其混合物。由于油的夹带，膜的颜色变得更深和褐色。与对照膜相比，夹油膜的表面越来越粗糙。共聚焦显微照片证实了油滴在生物聚合物网络内的均匀分布。在含柠檬醛的薄膜中估计出最大的微晶尺寸和最低的晶格应变。FTIR分析表明柠檬醛的掺入增加了膜基质中乳清蛋白分离物的β-折叠结构的比例。然而，含有香芹酚和柠檬醛组合的薄膜配方显示出最低的水蒸气透过率（WVTR），最高的拉伸强度，杨氏模量和破坏功。所有含油薄膜均显示出对引起细菌性胃肠炎模型细菌的良好抗菌作用，即蜡状芽孢杆菌和大肠杆菌。概括地说，可以得出结论，所制备的抗菌膜可以用于预防细菌性肠胃炎。