This study developed a novel method of glucose addition for the crack repair of concrete samples, which improved the bio-remediation efficiency. Various amounts of glucose were added to the medium at different sequence and the resulting absorbances were measured. Urea, calcium salt, and various amounts of glucose were mixed with an absorbance-fixed bacterial suspension and the resulting effect on calcium carbonate production was analyzed. Concrete specimens with irregular cracks were subjected to glucose addition for bio-remediation. Glucose addition decreased the pH, which inhibited the growth of bacteria. However, in a strong alkaline environment, this procedure was beneficial for crack repair. Informed by the bacterial growth and precipitation rates for calcium carbonate, addition of 10 g/L glucose was identified as the optimum concentration. After repair, the area recovery ratios of all samples exceeded 79%, while water penetration rates decreased significantly. The sonic time values of measurement points decreased and the strength of samples reached about 35% of the initial strength. Adding glucose to the medium during the repair improved the effect of the repair. This method can repair irregular cracks, accelerate the bio-remediation reaction, and reduce the repair time.

中文翻译：

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添加葡萄糖提高了裂缝修复的生物修复效率

本研究开发了一种新的葡萄糖添加方法用于混凝土样品的裂缝修复，提高了生物修复效率。以不同的顺序将不同量的葡萄糖加入培养基中，并测量所得的吸光度。尿素、钙盐和不同量的葡萄糖与吸光度固定的细菌悬浮液混合，分析对碳酸钙生产的影响。具有不规则裂缝的混凝土试件加入葡萄糖进行生物修复。添加葡萄糖会降低 pH 值，从而抑制细菌的生长。然而，在强碱性环境中，该程序有利于裂纹修复。根据碳酸钙的细菌生长和沉淀速率，确定添加 10 g/L 葡萄糖为最佳浓度。修复后，所有样品的面积恢复率均超过79%，而透水率显着下降。测量点声波时间值下降，样品强度达到初始强度的35%左右。在修复过程中向培养基中添加葡萄糖可提高修复效果。这种方法可以修复不规则裂缝，加速生物修复反应，减少修复时间。