In the last decades, there have been numerous reports about the separate interactions of a magnetic field and cold atmospheric plasma (CAP) with the biological systems. We have investigated the combined effect of CAP with the static magnetic field (SMF) as an effective method for cancer cells treatment. MDA - MB - 231 breast cancer cells were cultured and treated with CAP with different input power and exposure times in the presence and absence of the SMF. Vitamin C was also used in medium, and cell viability was investigated in the presence and absence of this antioxidant compound. The MTT assay was employed to measure cell survival, and a T -test or one-way ANOVA was used to assess the significance level of quantitative data. In order to determine the migration rate of cancer cells, wound healing assay was carried out. Results show that the presence of the SMF and vitamin C as well as increasing the input power significantly decrease the survival and migration rate of the cells. The results of the present investigation will greatly contribute to improve the CAP efficiency in cancer therapy by using the SMF and vitamin C as a complement to conventional CAP therapies.

中文翻译：

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使用维生素 C 和静态磁场提高冷大气压等离子体对乳腺癌细胞的控制能力和死亡率

在过去的几十年中，有许多关于磁场和冷大气等离子体 (CAP) 与生物系统单独相互作用的报告。我们研究了 CAP 与静磁场 (SMF) 的联合作用，作为治疗癌细胞的有效方法。MDA - MB - 231 乳腺癌细胞在存在和不存在 SMF 的情况下用不同的输入功率和曝光时间培养并用 CAP 处理。培养基中还使用了维生素 C，并在存在和不存在这种抗氧化剂化合物的情况下研究了细胞活力。采用 MTT 测定来测量细胞存活率，并使用 T 检验或单向方差分析来评估定量数据的显着性水平。为了确定癌细胞的迁移率，进行了伤口愈合试验。结果表明，SMF 和维生素 C 的存在以及增加输入功率显着降低了细胞的存活率和迁移率。本研究的结果将通过使用 SMF 和维生素 C 作为常规 CAP 疗法的补充，极大地有助于提高癌症治疗中的 CAP 效率。