Chemical cross-linking combined with mass spectrometry (XL-MS) and computational modeling has evolved as an alternative method to derive protein 3D structures and to map protein interaction networks. Special focus has been laid recently on the development and application of cross-linkers that are cleavable by collisional activation as they yield distinct signatures in tandem mass spectra. Building on our experiences with cross-linkers containing an MS-labile urea group, we now present the biuret-based, CID-MS/MS-cleavable cross-linker imidodicarbonyl diimidazole (IDDI) and demonstrate its applicability for protein cross-linking studies based on the four model peptides angiotensin II, MRFA, substance P, and thymopentin.

中文翻译：

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缩二脲衍生的，MS可裂解的交联剂，用于蛋白质结构分析：原理验证研究。

化学交联与质谱法（XL-MS）和计算模型的结合已发展成为一种衍生蛋白质3D结构和绘制蛋白质相互作用网络的替代方法。最近，人们特别关注交联剂的开发和应用，该交联剂可通过碰撞活化裂解，因为它们在串联质谱中产生不同的特征。基于我们对含有MS不稳定脲基的交联剂的经验，我们现在介绍基于缩二脲的CID-MS / MS可裂解的交联剂亚胺基二羰基二咪唑（IDDI），并证明其在基于蛋白质的交联研究中的适用性对四种模型肽血管紧张素II，MRFA，P物质和胸腺五肽有抑制作用。