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Gain and loss of a transcription factor that regulates late trichothecene biosynthetic pathway genes in Fusarium.
Fungal Genetics and Biology ( IF 3 ) Pub Date : 2019-12-10 , DOI: 10.1016/j.fgb.2019.103317
Daren W Brown 1 , Alessandra Villani 2 , Antonia Susca 2 , Antonio Moretti 2 , Guixia Hao 1 , Hye-Seon Kim 1 , Robert H Proctor 1 , Susan P McCormick 1
Affiliation  

Trichothecenes are among the mycotoxins of most concern to food and feed safety and are produced by species in two lineages of Fusarium: the F. incarnatum-equiseti (FIESC) and F. sambucinum (FSAMSC) species complexes. Previous functional analyses of the trichothecene biosynthetic gene (TRI) cluster in members of FSAMSC indicate that the transcription factor gene TRI6 activates expression of other TRI cluster genes. In addition, previous sequence analyses indicate that the FIESC TRI cluster includes TRI6 and another uncharacterized transcription factor gene (hereafter TRI21) that was not reported in FSAMSC. Here, gene deletion analysisindicated that in FIESC TRI6 functions in a manner similar to FSAMSC, whereas TRI21 activated expression of some genes that function late in the trichothecene biosynthetic pathway but not early-pathway genes. Consistent with this finding, TRI21 was required for formation of diacetoxyscripenol, a late-trichothecene-pathway product, but not for isotrichodermin, an early-pathway product. Although intact homologs of TRI21 were not detected in FSAMSC or other trichothecene-producing fungal genera, TRI21 fragments were detected in some FSAMSC species. This suggests that the gene was acquired by Fusarium after divergence from other trichothecene-producing fungi, was subsequently lost in FSAMSC, but was retained in FIESC. Together, our results indicate fundamental differences in regulation of trichothecene biosynthesis in FIESC and FSAMSC.

中文翻译:

镰刀菌中调节晚期天花粉体生物合成途径基因的转录因子的得失。

曲霉菌是最关注食品和饲料安全的霉菌毒素,由镰刀菌的两个谱系中的物种产生:镰刀菌(F. incarnatum-equiseti)(FIESC)和桑氏镰刀菌(F. sambucinum(FSAMSC)物种复合物。FSAMSC成员中的天花粉生物合成基因(TRI)簇的先前功能分析表明,转录因子基因TRI6激活了其他TRI簇基因的表达。此外,先前的序列分析表明,FIESC TRI簇包含TRI6和FSAMSC中未报道的另一个未表征的转录因子基因(以下称TRI21)。在这里,基因缺失分析表明在FIESC中TRI6以类似于FSAMSC的方式起作用,而TRI21激活了某些在单端孢菌素生物合成途径中起作用但在早期途径基因中不起作用的基因的表达。与这一发现相一致的是,TRI21是形成三乙酰基叔丁烯途径的产物二乙酰氧基隐烯醇的必需物质,而不是早期途径的异麦角菌素的形成所需要的。尽管未在FSAMSC或其他产生天花粉体的真菌属中检测到TRI21的完整同源物,但在某些FSAMSC物种中检测到了TRI21片段。这表明该基因在与其他产生天花粉的真菌发生分歧后被镰刀菌(Fusarium)收购,随后在FSAMSC中丢失,但在FIESC中保留了下来。总之,我们的结果表明FIESC和FSAMSC中天花粉烯生物合成调控的根本差异。尽管未在FSAMSC或其他产生天花粉体的真菌属中检测到TRI21的完整同源物,但在某些FSAMSC物种中检测到了TRI21片段。这表明该基因在与其他产生天花粉的真菌发生分歧后被镰刀菌(Fusarium)收购,随后在FSAMSC中丢失,但在FIESC中保留了下来。总之,我们的结果表明FIESC和FSAMSC中天花粉烯生物合成调控的根本差异。尽管未在FSAMSC或其他产生天花粉体的真菌属中检测到TRI21的完整同源物,但在某些FSAMSC物种中检测到了TRI21片段。这表明该基因在与其他产生天花粉的真菌发生分歧后被镰刀菌(Fusarium)收购,随后在FSAMSC中丢失,但在FIESC中保留了下来。总之,我们的结果表明FIESC和FSAMSC中天花粉烯生物合成调控的根本差异。
更新日期:2019-12-11
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