Leuconostoc mesenteroides includes the subsp. cremoris, subsp. dextranicum, subsp. mesenteroides and subsp. jonggajibkimchii, but the identification at the subspecies level using current phenotypic and/or genotypic methods is still difficult.

In this study, a polyphasic approach based on the analysis of rpoB gene polymorphism, Multiplex-PCR and phenotypic tests was optimised and used to identify a collection of Leuc. mesenteroides strains at the species and subspecies levels. The annotation of published Leuc. mesenteroides genomes was also revised.

A polymorphic region of rpoB gene was effective in separating Leuc. mesenteroides strains at the species (rpoB-species-specific-PCR) and subspecies (phylogenetic comparison) levels. Multiplex-PCR discriminated the subsp. mesenteroides from subsp. cremoris, but strains of uncertain attribution were found among subsp. dextranicum and subsp. jonggajibkimchii. Most of phenotypic features were not suitable for subspecies discrimination.

Our assays may provide a rapid and reliable identification of subsp. mesenteroides and subsp. cremoris strains in fermented foods. The discrimination of subsp. dextranicum and subsp. jonggajibkimchii suffered from several limitations (e.g. low number of available strains and genomes, phenotypic profile close to subsp. mesenteroides, discrepancy between genotypic and phenotypic traits) and further investigations are needed to clarify their delineation and taxonomical position.

mesenteroides包括在内。鸡骨草，亚种。dextranicum，亚种。mesenteroides和亚种。jonggajibkimchii，但是使用当前的表型和/或基因型方法进行亚种水平的鉴定仍然很困难。

在这项研究中，基于rpoB基因多态性分析，Multiple-PCR和表型测试的多相方法已得到优化，并用于鉴定Leuc的集合。mesenteroides菌株在物种和亚种水平上。已出版的Leuc的注释。mesenteroides基因组也进行了修订。

rpoB基因的多态性区域可有效分离Leuc。肠系膜在物种菌株（rpoB基因-species特异性-PCR）和亚种（系统发育比较）的水平。多重PCR区分了亚种。来自亚种的肠膜肠膜。creemoris，但亚种之间发现了不确定的归因菌株。葡聚糖和亚种。钟ga寺。大多数表型特征不适合亚种歧视。

我们的测定法可以提供快速，可靠的亚种鉴定。mesenteroides和亚种。发酵食品中的cremoris菌株。对亚种的区分。葡聚糖和亚种。宗加吉布基米菌有几个局限性（例如，可用菌株和基因组数量少，接近中肠膜亚种的表型分布，基因型和表型性状之间的差异），还需要进一步的研究来阐明它们的轮廓和分类学位置。