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Nanomolar detection of biothiols via turn-ON fluorescent indicator displacement.
Analyst ( IF 4.2 ) Pub Date : 2019-12-17 , DOI: 10.1039/c9an02222h P P Praveen Kumar 1 , Navneet Kaur , Asifkhan Shanavas , Prakash P Neelakandan
Analyst ( IF 4.2 ) Pub Date : 2019-12-17 , DOI: 10.1039/c9an02222h P P Praveen Kumar 1 , Navneet Kaur , Asifkhan Shanavas , Prakash P Neelakandan
Affiliation
The thiol group is known to have a high affinity for gold nanoparticles and is capable of displacing other capping agents. We report a fluorescent indicator displacement technique for the detection of biothiols based on this principle. The displacement of BODIPY from the surface of gold nanoparticles served as a sensitive method for the detection of cysteine, homocysteine, and glutathione under a variety of conditions. It is noteworthy that the turn-ON fluorescence mechanism enabled the differentiation of normal cells from cancer cells because of the inherently higher concentrations of biothiols in the latter.
中文翻译:
通过开启荧光指示剂位移对生物硫醇进行纳摩尔检测。
已知巯基对金纳米颗粒具有高亲和力,并且能够取代其他封端剂。我们报告了基于此原理的生物指示剂检测荧光指示剂置换技术。BODIPY从金纳米颗粒表面的置换是在各种条件下检测半胱氨酸,高半胱氨酸和谷胱甘肽的灵敏方法。值得注意的是,开启荧光机制使正常细胞从癌细胞中分化出来,因为后者中固有的生物硫醇浓度较高。
更新日期:2020-02-13
中文翻译:
通过开启荧光指示剂位移对生物硫醇进行纳摩尔检测。
已知巯基对金纳米颗粒具有高亲和力,并且能够取代其他封端剂。我们报告了基于此原理的生物指示剂检测荧光指示剂置换技术。BODIPY从金纳米颗粒表面的置换是在各种条件下检测半胱氨酸,高半胱氨酸和谷胱甘肽的灵敏方法。值得注意的是,开启荧光机制使正常细胞从癌细胞中分化出来,因为后者中固有的生物硫醇浓度较高。