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A novel eIF4E-interacting protein that forms non-canonical translation initiation complexes.
Nature Plants ( IF 18.0 ) Pub Date : 2019-12-09 , DOI: 10.1038/s41477-019-0553-2
René Toribio 1 , Alfonso Muñoz 1, 2 , Ana B Castro-Sanz 1 , Catharina Merchante 3 , M Mar Castellano 1
Affiliation  

Translation is a fundamental step in gene expression that regulates multiple developmental and stress responses. One key step of translation initiation is the association between eIF4E and eIF4G. This process is regulated in different eukaryotes by proteins that bind to eIF4E; however, evidence of eIF4E-interacting proteins able to regulate translation is missing in plants. Here, we report the discovery of CERES, a plant eIF4E-interacting protein. CERES contains an LRR domain and a canonical eIF4E-binding site. Although the CERES-eIF4E complex does not include eIF4G, CERES forms part of cap-binding complexes, interacts with eIF4A, PABP and eIF3, and co-sediments with translation initiation complexes in vivo. Moreover, CERES promotes translation in vitro and general translation in vivo, while it modulates the translation of specific mRNAs related to light and carbohydrate response. These data suggest that CERES is a non-canonical translation initiation factor that modulates translation in plants.

中文翻译:

一种形成非规范翻译起始复合物的新型 eIF4E 相互作用蛋白。

翻译是调节多种发育和压力反应的基因表达的基本步骤。翻译起始的一个关键步骤是 eIF4E 和 eIF4G 之间的关联。这个过程在不同的真核生物中受到与 eIF4E 结合的蛋白质的调节;然而,植物中缺少能够调节翻译的 eIF4E 相互作用蛋白的证据。在这里,我们报告了 CERES,一种植物 eIF4E 相互作用蛋白的发现。CERES 包含一个 LRR 域和一个规范的 eIF4E 结合位点。尽管 CERES-eIF4E 复合物不包括 eIF4G,但 CERES 是帽结合复合物的一部分,与 eIF4A、PABP 和 eIF3 相互作用,并在体内与翻译起始复合物共沉淀。此外,CERES 促进体外翻译和体内一般翻译,同时它调节与光和碳水化合物反应相关的特定 mRNA 的翻译。这些数据表明 CERES 是调节植物翻译的非规范翻译起始因子。
更新日期:2019-12-11
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