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Environmentally-safe scCO2 P. pinaster branches extracts: Composition and properties
Journal of CO2 Utilization ( IF 7.7 ) Pub Date : 2019-12-10 , DOI: 10.1016/j.jcou.2019.11.027
Marisa C. Gaspar , Hermínio C. de Sousa , Inês J. Seabra , Mara E.M. Braga

Considering the vast array of compounds that plants manufacture that help protect them against insects’ attacks, plant raw materials are a potential source of future insecticides/larvicides. In this work, volatiles from maritime pine (Pinus pinaster) branches, a pruning residue, were extracted by supercritical carbon dioxide (scCO2), and also by hydrodistillation for comparison purposes. The volatiles naturally emitted from the pine branches were identified as well. Experimental design results showed higher extraction yields (up to 6%), when scCO2 density and temperature increased, while the inverse occurred for total phenolic contents (1.2–3.3% GAE, gallic acid equivalents). Emitted volatiles from the branches were mainly α-pinene, β-pinene, β-myrcene, limonene, and β-caryophyllene. In general, the volatile profiles of the scCO2 and hydrodistillation extracts were quite similar, revealing abietadiene, the precursor of abietic acid, as the main compound. Oxidation inhibitions of ∼42 – 59% were obtained for the scCO2 extracts in the β-carotene assay. The acetylcholinesterase inhibitory activity assay revealed the potential of these extracts to have insecticidal/larvicidal activity, with IC50 values of 2.8–10.7 mg/mL. Overall, these extracts may be applied in environmentally-safe insect/larvae control strategies, in formulations that benefit from the presence of antioxidants.



中文翻译:

环保型scCO 2 P. pinaster分支提取物:组成和性质

考虑到植物生产的多种化合物可帮助保护它们免受昆虫的侵袭,植物原料是未来杀虫剂/杀幼虫剂的潜在来源。在这项工作中,通过超临界二氧化碳(scCO 2)以及加氢蒸馏法提取了海松(Pinus pinaster)树枝中的修剪残留物,并进行了加氢蒸馏以进行比较。还确定了从松枝自然释放的挥发物。实验设计结果表明,当scCO 2达到更高的提取率时(高达6%)密度和温度增加,而总酚含量(1.2-3.3%GAE,没食子酸当量)则相反。分支中散发的挥发物主要是α-pine烯,β-pine烯,β-月桂烯,li烯和β-石竹烯。通常,scCO 2和加氢蒸馏提取物的挥发物特征非常相似,表明松香酸的前体阿比他二烯为主要化合物。在β-胡萝卜素测定中,scCO 2提取物的氧化抑制率为〜42 – 59%。乙酰胆碱酯酶抑制活性试验表明,这些提取物具有杀虫/杀幼虫活性,IC 50值2.8-10.7 mg / mL。总体而言,这些提取物可用于环境安全的昆虫/幼虫控制策略中,其配方可受益于抗氧化剂的存在。

更新日期:2019-12-11
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