Abstract Sweet-waxy corn is susceptible to chilling injury, and vulnerable to be counterfeited. This study developed a kind of newly thermoresponsive coating material with dual efficacies of intelligent chilling-resistant and anti-counterfeiting for maize seed. The coating material was named as PRBSA, which was made of salicylic acid (SA) and rhodamine B (RB) co-loaded poly (N-isopropylacrylamide-co-butylmethacrylate) hydrogel. The surface morphology of PRBSA was compact than its interior, where it was uneven and irregularly distributed pore structure. Below the lower critical solution temperature (LCST), PRBSA was water-soluble and hydrophilic, loaded SA and RB could release sharply from PRBSA, whereas above this LCST, PRBSA formed an insoluble and hydrophobic aggregate, the release rate of SA and RB was obviously decreased. Meanwhile, PRBSA showed a better thermal stability than unloaded hydrogel. In addition, seeds were coated with PRBSA, and its seedling quality and fluorescence labeling under chilling stress were determined. These results showed that compared with the CK (basic coating agent), PRBSA treatment significantly enhanced seed germination percentage by 17.8% and vigor index by 53.1% under chilling stress. The seedling shoot height and dry weight were also significantly improved, which might closely related with the lowest malondialdehyde and relative electrical conductivity level, and the highest maximal quantum yield of PSII (Fv/Fm). Moreover, the surface of PRBSA coated seeds showed bright red fluorescence under the excitation green light of 546 nm. When responding to low temperatures, its fluorescence intensity exhibited a sharp decline, showing a temperature-sensitive release of RB. Therefore, PRBSA was considered as a novel seed coating with intelligent chilling-resistant and anti-counterfeiting function.

中文翻译：

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高分子水凝胶抗寒防伪热敏玉米种子包衣剂的制备

摘要 甜糯玉米易受冷害，易被仿冒。本研究开发了一种具有智能抗冷防伪双重功效的新型玉米种子热敏包覆材料。该涂层材料命名为PRBSA，由水杨酸（SA）和罗丹明B（RB）共载聚（N-异丙基丙烯酰胺-共-甲基丙烯酸丁酯）水凝胶制成。PRBSA的表面形态比其内部更致密，内部是不均匀且不规则分布的孔隙结构。在下临界溶解温度 (LCST) 以下，PRBSA 是水溶性和亲水性的，负载的 SA 和 RB 可以从 PRBSA 中快速释放，而在此 LCST 以上，PRBSA 形成不溶性和疏水性聚集体，SA 和 RB 的释放速率明显减少了。同时，PRBSA 显示出比卸载水凝胶更好的热稳定性。此外，用PRBSA包被种子，测定其在低温胁迫下的幼苗质量和荧光标记。这些结果表明，与CK（碱性包衣剂）相比，PRBSA处理显着提高了低温胁迫下种子发芽率17.8%和活力指数53.1%。苗高和干重也显着提高，这可能与最低的丙二醛和相对电导率水平以及最高的PSII最大量子产率（Fv/Fm）密切相关。此外，PRBSA包被的种子表面在546 nm激发绿光下呈现亮红色荧光。当响应低温时，其荧光强度表现出急剧下降，显示 RB 的温度敏感释放。因此，PRBSA被认为是一种具有智能防寒防伪功能的新型种衣剂。