Ubiquitination is involved in synaptic plasticity and memory, but the involvement of HECT E3 ligases in these processes has not yet been established. Here, we bilaterally infused heclin, a specific inhibitor of some of these ligases, into the dorsal hippocampus of male Wistar rats that were trained in a contextual fear conditioning. Heclin improved short-term memory, consolidation, retrieval, and reconsolidation when administered immediately post training, prior to testing, or after memory reactivation, respectively. In addition, it impaired memory extinction when administered prior to a long reactivation session. Heclin infusion was also tested for locomotor activity and anxiety-like behavior in a circular arena, but no effect was seen. Taken together, these results indicate that HECT E3 ligases are involved in the modulation of fear memory.

中文翻译：

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海马HECT E3连接酶抑制促进整合，恢复和再整合，并抑制背景恐惧记忆的消失。

泛素化参与突触可塑性和记忆，但尚未确定HECT E3连接酶在这些过程中的参与。在这里，我们将海克林（一种其中的某些连接酶的特异性抑制剂）双向注入到接受情境恐惧条件训练的雄性Wistar大鼠的背侧海马中。当分别在训练后，测试之前或记忆重新激活后立即给予Heclin时，Heclin改善了短期记忆，巩固，恢复和重新巩固。此外，如果在长时间的重新激活之前进行给药，则会损害记忆的消失。还测试了Heclin输注液在圆形竞技场中的运动活性和焦虑样行为，但未见效果。两者合计，这些结果表明，HECT E3连接酶参与恐惧记忆的调制。