Lysosomotropic agents have been used to permeabilize lysosomes and thereby implicate these organelles in diverse cellular processes. Since lysosomes are Ca2+ stores, this rupturing action, particularly that induced by GPN, has also been used to rapidly release Ca2+ from lysosomes. However, a recent study has questioned the mechanism of action of GPN and concluded that, acutely, it does not permeabilize lysosomes but releases Ca2+ directly from the ER instead. We therefore appraise these provocative findings in the context of the existing literature. We suggest that further work is required to unequivocally rule out lysosomes as contributors to GPN-evoked Ca2+ signals.

中文翻译：

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溶酶体破裂会引起Ca2 +释放吗？毛孔和存储的问题。

溶溶亲和剂已被用于使溶酶体通透，从而使这些细胞器参与多种细胞过程。由于溶酶体是Ca2 +的储存体，这种破裂作用，尤其是由GPN诱导的破裂作用，也已被用来从溶酶体中快速释放Ca2 +。但是，最近的一项研究对GPN的作用机理提出了质疑，并得出结论：急性地，它不能渗透溶酶体，而是直接从ER中释放Ca2 +。因此，我们在现有文献的背景下评估这些令人鼓舞的发现。我们建议需要进一步的工作，以明确排除溶酶体作为促成GPN引起的Ca2 +信号的原因。