Spring viremia of carp virus (SVCV) is one of the most serious pathogens in aquaculture, resulting in devastating damage in cyprinid. In this study, we designed and synthesized a novel coumarin derivative (C3007) for evaluating its in vitro and in vivo anti-SVCV effects. Here, we determined that up to 25 mg/L C3007 significantly decreased SVCV protein gene expression levels in EPC cells by a maximum inhibitory rate of >95%. When C3007 was preincubated with SVCV, infectivity was significantly inhibited in vitro in a time-dependent manner, with complete inhibition at 25 mg/L. For in vivo studies, C3007 exhibited an anti-SVCV effect by substantially enhancing the survival rate of virus-infected fish via intraperitoneal injection. Although the horizontal transmission of SVCV was hindered by C3007 in a static cohabitation challenge model, it was not completely blocked, showing that the viral loads in recipient fish were obviously reduced. Thus, C3007 could potentially be used as a therapeutic agent with great potential in aquatic systems and may also be suitable for applications in pond aquaculture settings against viral transmission. Additionally, the C3007-preincubated virus induced an antiviral immune response with high levels of IFN expression, suggesting that C3007 pre-treatment could be used in vaccine development.

中文翻译：

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通过靶向病毒感染的早期对水生弹状病毒抑制新型香豆素证明了其在水产养殖中的潜在应用。

鲤鱼病毒的春季病毒血症（SVCV）是水产养殖中最严重的病原体之一，导致塞浦路斯的毁灭性破坏。在这项研究中，我们设计和合成了一种新型香豆素衍生物（C3007），用于评估其体内和体外的抗SVCV效果。在这里，我们确定高达25 mg / L的C3007可以显着降低EPC细胞中SVCV蛋白质基因的表达水平，最大抑制率> 95％。当将C3007与SVCV预孵育时，感染性在体外以时间依赖性方式被显着抑制，在25 mg / L时完全被抑制。对于体内研究，C3007通过腹膜内注射显着提高病毒感染鱼的存活率而表现出抗SVCV效果。尽管在静态同居挑战模型中C3007阻碍了SVCV的水平传播，它并没有完全被阻止，表明受体鱼中的病毒载量明显减少了。因此，C3007可以潜在地用作在水生系统中具有巨大潜力的治疗剂，并且也可能适合在池塘水产养殖设施中应用以对抗病毒传播。另外，C3007预孵育的病毒诱导了具有高水平IFN表达的抗病毒免疫反应，表明C3007预处理可用于疫苗开发。