当前位置: X-MOL 学术Mol. Cell. Endocrinol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Relevant dose of the environmental contaminant, tributyltin, promotes histomorphological changes in the thyroid gland of male rats.
Molecular and Cellular Endocrinology ( IF 4.1 ) Pub Date : 2019-12-09 , DOI: 10.1016/j.mce.2019.110677
Paula Rodrigues-Pereira 1 , Sofia Macedo 2 , Tiago Bordeira Gaspar 2 , Sule Canberk 2 , Samia Selmi-Ruby 3 , Valdemar Máximo 4 , Paula Soares 4 , Leandro Miranda-Alves 5
Affiliation  

Organotin compounds, such as tributyltin (TBT), are common environmental contaminants and suspected endocrine-disrupting chemicals. Tributyltin is found in antifouling paints, widely used in ships and other vessels. The present study evaluated whether a 15-day treatment with TBT at a dose of 100 ng/kg/day could induce histomorphological changes in the thyroid gland of rats. TBT promoted relevant alterations in the thyroid architecture, being the most relevant histological findings the presence of increased number of small-size follicles in the treated group. In qualitative analyses, colloid vacuolization, papillary budging structures, cystic degeneration and chronic thyroiditis, were observed. Moreover, histomorphometric analysis showed statistically significant changes in the follicular architecture of TBT-treated rats, mainly a decrease in the follicle area (colloid) and an increased epithelial height that resulted in an increased epithelial height/colloid ratio. Augmented collagen deposition was also seen in the thyroids of treated groups. In immunohistochemical (IHC) analyses, the localization of NIS protein was described and a significant increased proliferation index (evaluated by Ki67 positive cells) in the treated group was reported. As an indirect measurement of oxidative stress, mitochondrial protein SDHA was also analyzed by IHC analysis. Although the cytoplasmic expression of SDHA was observed in both groups, the staining intensity score was higher in TBT-treated group. Our results suggest that besides causing histomorphological changes, environmental relevant dose of TBT treatment can also induce oxidative alterations.

中文翻译:

相关剂量的环境污染物三丁锡可促进雄性大鼠甲状腺的组织形态学变化。

有机锡化合物,例如三丁基锡(TBT),是常见的环境污染物和可疑破坏内分泌的化学物质。三丁基锡存在于防污涂料中,广泛用于船舶和其他船舶。本研究评估了以100 ng / kg /天的剂量使用TBT进行15天治疗是否可以诱导大鼠甲状腺的组织形态学变化。TBT促进了甲状腺结构的相关改变,这是最相关的组织学发现,治疗组中存在小卵泡数量增加。在定性分析中,观察到胶体空泡,乳头芽结构,囊性变性和慢性甲状腺炎。此外,组织形态计量学分析显示,经TBT处理的大鼠的卵泡结构具有统计学上的显着变化,主要是卵泡面积的减少(胶体)和上皮高度的增加,导致上皮高度/胶体比的增加。在治疗组的甲状腺中也观察到胶原蛋白沉积增加。在免疫组化(IHC)分析中,描述了NIS蛋白的定位,并报告了治疗组中增殖指数显着提高(通过Ki67阳性细胞评估)。作为间接测定氧化应激的方法,也通过IHC分析法分析了线粒体蛋白SDHA。尽管在两组中均观察到SDHA的细胞质表达,但是在TBT处理的组中染色强度评分较高。我们的结果表明,除引起组织形态变化外,与环境有关的剂量的TBT处理剂量还可以诱导氧化变化。
更新日期:2019-12-09
down
wechat
bug