Titanium (Ti) and its alloys are widely used in clinical practice as preferred materials for bone tissue repair and replacement because of their good mechanical properties; however, as Ti lacks biological activity, clinical application has been limited. Herein, we prepared a manganese–titanium dioxide (Mn–TiO₂) microporous biotic coating on Ti surfaces by micro-arc oxidation (MAO). The coating showed good surface topography and was uniformly doped with Mn, and the Mn ions were slowly released. In vitro, the Mn–TiO₂ microporous biotic coating promoted the adhesion, proliferation, differentiation, and mineralization of MC3T3-E1 osteoblasts. Moreover, in vivo experiments showed that the coating promoted early osseointegration. We also conducted a preliminary investigation to explore the molecular mechanism underlying the regulation of the function of osteoblasts by the coating. Furthermore, we found that the coating could inhibit the growth of Escherichia coli in vitro, demonstrating reliable antibacterial ability. To conclude, Mn–TiO₂ microporous biotic coating can improve the biological activity of Ti implants, which can potentially improve their clinical applications.

钛及其合金由于其良好的机械性能而在临床实践中广泛用作骨组织修复和置换的首选材料。但是，由于钛缺乏生物活性，因此临床应用受到限制。在这里，我们通过微弧氧化（MAO）在Ti表面制备了锰-二氧化钛（Mn-TiO ₂）微孔生物涂层。涂层显示出良好的表面形貌，并均匀地掺杂了Mn，并且Mn离子被缓慢释放。在体外，Mn–TiO ₂微孔生物涂层促进了MC3T3-E1成骨细胞的粘附，增殖，分化和矿化。而且，体内实验表明，涂层促进了早期骨整合。我们还进行了初步研究，以探索通过涂层调节成骨细胞功能的分子机制。此外，我们发现该涂层可以抑制大肠杆菌的体外生长，显示出可靠的抗菌能力。总之，Mn–TiO ₂微孔生物涂层可以改善Ti植入物的生物活性，从而有可能改善其临床应用。