Protein phosphatase 6 (PP6) is a member of the PP2A-like subfamily, which plays a critical role in many fundamental cellular processes. We recently reported that PP6 is essential for female fertility. Here, we report that PP6 is involved in meiotic recombination and that germ cell-specific deletion of PP6 by Stra8-Cre causes defective spermatogenesis. The PP6-deficient spermatocytes were arrested at the pachytene stage and defects in DSB repair and crossover formation were observed, indicating that PP6 facilitated meiotic double-stranded breaks (DSB) repair. Further investigations revealed that depletion of PP6 in the germ cells affected chromatin relaxation, which was dependent on MAPK pathway activity, consequently preventing programmed DSB repair factors from being recruited to proper positions on the chromatin. Taken together, our results demonstrate that PP6 has an important role in meiotic recombination and male fertility.

中文翻译：

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蛋白磷酸酶 6 是调节精子发生的关键因素。

蛋白磷酸酶 6 (PP6) 是 PP2A 样亚家族的成员，在许多基本细胞过程中发挥着关键作用。我们最近报道了 PP6 对女性生育能力至关重要。在这里，我们报告 PP6 参与减数分裂重组，并且 Stra8-Cre 对 PP6 的生殖细胞特异性缺失导致精子发生缺陷。PP6 缺陷型精母细胞在粗线期停滞，并观察到 ​​DSB 修复和交叉形成缺陷，表明 PP6 促进了减数分裂双链断裂 (DSB) 修复。进一步的研究表明，生殖细胞中 PP6 的消耗会影响染色质松弛，这取决于 MAPK 途径的活性，从而阻止程序化的 DSB 修复因子被招募到染色质上的适当位置。综合起来，