Juvenile hormone binding proteins (JHBPs) serve as the carriers that transport juvenile hormone (JH) to target tissues to activate JH signals. In this study, seven JHBPs from mud crab Scylla paramamosain were obtained. All Sp-JHBPs contained one JHBP domain, and Sp-JHBP4, 5, 6 also contained one Grp7_allergen domain. The predicted secondary and 3D structures are conserved among the seven Sp-JHBP domains, but the complete 3D structure of Sp-JHBP4, 5, 6 were more similar to lipopolysaccharide binding protein. Additionally, the potential ligands for Sp-JHBP1, 2, 3, 7 were ubiquinone 8 (UQ8) and JH II or JH III, and the predicted binding location and sites for JHs were similar to the structured known BmJHBP, suggesting that MF is a potential ligand for these Sp-JHBPs. Furthermore, the expression analysis showed that Sp-JHBP1 expression is relatively low in the 14 detected tissues, and Sp-JHBP2 has the highest expression in the ovary, while the other five JHBPs are highly expressed in the hepatopancreas. During larval development, Sp-JHBP2 is highly expressed during the Z1, M and C1 stages, which are three post-metamorphosis stages, while Sp-JHBP3 and 4 expression levels were significantly lower during the Z5 and M stages, which are two pre-metamorphosis stages. Moreover, the in vivo and in vitro study revealed that Sp-JHBP2 expression was reduced by MF, while the in vitro studies showed that Sp-JHBP3, 6, 7 are induced by MF in a concentration-independent manner. Taken together, we hypothesized Sp-JHBP1, 2, 3, 7 has the potential to bind MF and Sp-JHBP2, 3, 7 play a more important roles in MF signal, while Sp-JHBP4, 5, 6 were more likely to participate in immune response.

中文翻译：

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泥蟹Scylla paramamosain中的七个推定JHBP的表征和表达分析：与法呢酸甲酯的推定关系。

少年激素结合蛋白（JHBP）作为将少年激素（JH）转运到靶组织以激活JH信号的载体。在这项研究中，从泥蟹Sylla paramamosain获得了七个JHBP。所有的Sp-JHBPs都含有一个JHBP结构域，而Sp-JHBP4、5、6也都含有一个Grp7_allergen结构域。预测的二级和3D结构在七个Sp-JHBP域之间是保守的，但Sp-JHBP4、5、6的完整3D结构与脂多糖结合蛋白更为相似。此外，Sp-JHBP1、2、3、7的潜在配体是泛醌8（UQ8）和JH II或JH III，并且预测的JHs结合位置和位点类似于结构化的已知BmJHBP，这表明MF是一种这些Sp-JHBP的潜在配体。此外，表达分析表明，在检测到的14个组织中Sp-JHBP1表达相对较低，而Sp-JHBP2在卵巢中表达最高，而其他5个JHBP在肝胰腺中高表达。在幼虫发育期间，Sp-JHBP2在变态后的三个阶段Z1，M和C1阶段高表达，而在Z5和M的两个阶段，Sp-JHBP3和4的表达水平显着降低。变态阶段。此外，体内和体外研究表明，MF降低了Sp-JHBP2的表达，而体外研究表明，MF以非浓度依赖性的方式诱导了Sp-JHBP3、6、7。综上所述，我们假设Sp-JHBP1、2、3、7具有结合MF的潜力，而Sp-JHBP2、3、7在MF信号中起着更重要的作用，而Sp-JHBP4、5