Microalgae are a sustainable energy source because of their lipid storage capacity. However, the cultivation of microalgae requires optimization to produce a high biomass and lipid content. Stressing the cell is a common approach that is applied to increase the lipid content of the algae. Within this context, sodium chloride (NaCl) stress and magnesium (Mg²⁺) limitations were applied individually and mutually to understand their potential effects on biomass, lipid quantity, and the fatty acid methyl esters (FAME) profile of Auxenochlorella protothecoides. Saturated fatty acid (SFA) contents as high as 43.9% were obtained at 0 mg L⁻¹ Mg²⁺ with 5 g L⁻¹ NaCl. The highest linoleic acid content (35.2%) was obtained at 0.3 mg L⁻¹ Mg²⁺ with 2.5 g L⁻¹ NaCl. However, the highest biomass was achieved at 18.5 mg L⁻¹ Mg²⁺ with 5.0 g L⁻¹ NaCl according to surface response methodology. These optimization data could be useful for producing a feasible and sustainable biodiesel production strategy with high biomass and lipid productivity.

微藻由于其脂质存储能力而成为可持续的能源。然而，微藻的培养需要优化以产生高生物质和脂质含量。向细胞施加压力是用于增加藻类脂质含量的常用方法。在此背景下，分别和相互应用了氯化钠（NaCl）胁迫和镁（Mg ²⁺）限制，以了解它们对原栖小球藻的生物量，脂质量和脂肪酸甲酯（FAME）谱的潜在影响。在0 mg L ^-1 Mg ²⁺和5 g L ^{-1的条件下，}饱和脂肪酸（SFA）含量高达43.9％氯化钠 在具有2.5 g L ^-1 NaCl的0.3 mg L ^-1  Mg ²⁺处获得最高的亚油酸含量（35.2％）。然而，根据表面响应方法，在最高生物量为18.5 mg L ^-1  Mg ²⁺和5.0 g L ^-1 NaCl的情况下。这些优化数据可用于产生具有高生物量和脂质生产率的可行且可持续的生物柴油生产策略。