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A paper-based SERS assay for sensitive duplex cytokine detection towards the atherosclerosis-associated disease diagnosis.
Journal of Materials Chemistry B ( IF 7 ) Pub Date : 2020-04-29 , DOI: 10.1039/c9tb02469g
Chunxia Li 1 , Yuan Liu , Xiaoyan Zhou , Yuling Wang
Affiliation  

Atherosclerosis (AS) is the most common factor causing many cardiovascular and cerebrovascular diseases and has received considerable attention. The occurrence mechanism of AS is uncertain because it is a choronically pathological process that is influenced by multi-aspects, among which cytokines play the key roles in regulating the processes of the immune system. For example, two key cytokines, namely, IL-10 and MCP-1 (chemokine), which are involved in AS progression with varied levels, can be used for AS status monitoring and early diagnosis of AS-associated diseases. Hence, a new paper-based, surface-enhanced Raman spectroscopy (SERS) sensing platform was established for the detection of these two key cytokines. By combining a nanoporous networking membrane as the substrate and SERS nanotags as the probe for signal reading, together with a sandwich design, sensitive and specific identification and quantification of cytokine targets in human serum were achieved with excellent sensing characteristics. The lowest detectable concentration was determined to be 0.1 pg mL-1 for both IL-10 and MCP-1 in human serum. The assay also exhibits high specificity towards target cytokine detection, with low-nonspecific binding and acceptable cross-reactivity in the presence of other structurally similar targets. Finally, the practicability was validated by performing duplex detection in human serum, which further demonstrates the high specificity of the assay for the detection of target cytokines. Taken together, these promising results illustrate that this developed sensing assay is a candidate for clinical multi-target analysis in real environments.

中文翻译:

一种基于纸质的SERS检测方法,用于敏感的双工细胞因子检测,可用于动脉粥样硬化相关疾病的诊断。

动脉粥样硬化(AS)是导致许多心脑血管疾病的最常见因素,受到了广泛的关注。AS的发生机制是不确定的,因为它是受多种因素影响的慢性病理过程,其中细胞因子在调节免疫系统的过程中起关键作用。例如,可以以不同水平参与AS进展的两种关键细胞因子IL-10和MCP-1(趋化因子)可用于AS状态监测和AS相关疾病的早期诊断。因此,建立了一个新的基于纸张的表面增强拉曼光谱(SERS)传感平台,用于检测这两种关键细胞因子。通过结合纳米多孔网络膜作为底物和SERS纳米标签作为信号读取的探针,结合三明治设计,可实现具有出色传感特性的人血清中细胞因子靶标的灵敏,特异的鉴定和定量。测定人血清中IL-10和MCP-1的最低可检测浓度为0.1 pg mL-1。该测定法还显示出对靶细胞因子检测的高特异性,在存在其他结构相似靶标的情况下具有低的非特异性结合和可接受的交叉反应性。最后,通过在人血清中进行双重检测验证了实用性,这进一步证明了该检测方法对靶细胞因子检测的高度特异性。综上所述,这些令人鼓舞的结果表明,这种开发的感测方法可在实际环境中进行临床多目标分析。具有出色的传感特性,实现了对人血清中细胞因子靶标的灵敏,特异的鉴定和定量。测定人血清中IL-10和MCP-1的最低可检测浓度为0.1 pg mL-1。该测定法还显示出对靶细胞因子检测的高特异性,在其他结构相似的靶存在下具有低的非特异性结合和可接受的交叉反应性。最后,通过在人血清中进行双重检测验证了实用性,这进一步证明了该检测方法对靶细胞因子检测的高度特异性。综上所述,这些令人鼓舞的结果表明,这种开发的感测方法可在实际环境中进行临床多目标分析。具有出色的传感特性,实现了对人血清中细胞因子靶标的灵敏,特异的鉴定和定量。测定人血清中IL-10和MCP-1的最低可检测浓度为0.1 pg mL-1。该测定法还显示出对靶细胞因子检测的高特异性,在存在其他结构相似靶标的情况下具有低的非特异性结合和可接受的交叉反应性。最后,通过在人血清中进行双重检测验证了实用性,这进一步证明了该检测方法对靶细胞因子检测的高度特异性。综上所述,这些令人鼓舞的结果表明,这种开发的感测方法可在实际环境中进行临床多目标分析。
更新日期:2019-12-06
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