In a study of the potential anti-inflammatory constituents from Kalimeris shimadae, six new sesquiterpenes, kalshinoids A-F (1-6), together with 21 known compounds (7-27), were isolated. The structures and absolute configurations of the new compounds were discerned from extensive spectroscopic analysis, and the absolute configurations of kalshinoids A, B, E, and F were established by ECD calculations. Furthermore, the identified compounds were tested for anti-inflammatory activity as assessed by inhibition of tumor necrosis factor-alpha (TNF-α) in THP-1 cells. Three sesquiterpenes [kalshinoid F, 4(15)-eudesmen-1β,7,11-triol, and 4α,10α,11-trihydroxy-1βH,5βH-guai-7(8)-ene] reduced levels of TNF-α in lipopolysaccharide-stimulated THP-1 cells in a concentration-dependent manner and were more potent than dexamethasone. These natural sesquiterpenes merit further investigation as possible anti-inflammatory agents.

中文翻译：

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Kalshinoids AF，来自Kalimeris shimadae的抗炎倍半萜烯。

在一项研究中发现了来自Kalimeris shimadae的潜在抗炎成分，分离出了六种新的倍半萜，kalshinoids AF（1-6）以及21种已知化合物（7-27）。从广泛的光谱分析中可以辨别出新化合物的结构和绝对构型，并通过ECD计算确定了碱类化合物A，B，E和F的绝对构型。此外，通过抑制THP-1细胞中的肿瘤坏死因子-α（TNF-α）评估了鉴定出的化合物的抗炎活性。三种倍半萜[kalshinoid F，4（15）-eudesmen-1β，7,11-triol和4α，10α，11-trihydroxy-1βH，5βH-guai-7（8）-ene]降低TNF-α的水平脂多糖刺激的THP-1细胞具有浓度依赖性，并且比地塞米松更有效。