In this paper, we proposed a novel approach for rapid and flexible fabrication of self-driven microfluidic surface enhanced Raman scattering (SERS) chips for quantitative analysis of Hg2+ by femtosecond laser direct writing. In contrast to traditional microfluidic chips, the microchannels of the device can drive a liquid sample flow without external driving force. The sample flow speed is tunable since the wettability and capillarity properties of the channels, which depend on the roughness and the inner diameter of the microchannels, can be controlled by optimizing the laser processing parameters. The SERS active detection sites, which exhibit high enhancement effects and fine reproducibility, were integrated through the femtosecond laser-induced periodic surface structures (LIPSS), followed by 30 nm Ag deposition. The SERS performance of the as-prepared microfluidic SERS detection chip was studied with R6G as probe molecules. The quantitative analysis of Hg2+ was realized by simply injecting the Hg2+ sample and the probe molecules R6G from the two inlets, separately, and collecting the SERS signal at the detection site. The lowest detection limit for Hg2+ is 10-9 M. It should be mentioned that this study is not only limited to Hg2+ quantitative analysis, but is also mainly aimed to develop a new technique for the design and fabrication of novel self-driven microfluidic devices depending on practical application requirements.

中文翻译：

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飞秒激光制造的Hg2 +检测用自驱动微流控表面增强拉曼散射装置。

在本文中，我们提出了一种新的方法来快速灵活地制造自驱动微流控表面增强拉曼散射（SERS）芯片，用于通过飞秒激光直接写入对Hg2 +进行定量分析。与传统的微流控芯片相比，该设备的微通道可以在没有外部驱动力的情况下驱动液体样品流。样品流速是可调的，因为可以通过优化激光加工参数来控制取决于微通道的粗糙度和内径的通道的润湿性和毛细特性。通过飞秒激光诱导的周期性表面结构（LIPSS），然后通过30 nm Ag沉积，整合了具有高增强作用和良好重现性的SERS活性检测位点。以R6G为探针分子研究了所制备的微流控SERS检测芯片的SERS性能。只需分别从两个入口分别注入Hg2 +样品和探针分子R6G，并在检测部位收集SERS信号，即可对Hg2 +进行定量分析。Hg2 +的最低检出限为10-9M。应该指出的是，本研究不仅限于Hg2 +定量分析，而且主要目的是开发一种用于设计和制造新型自驱动微流控装置的新技术。视实际应用需求而定。并在检测点收集SERS信号。Hg2 +的最低检出限为10-9M。应该指出的是，本研究不仅限于Hg2 +定量分析，而且主要目的是开发一种用于设计和制造新型自驱动微流控装置的新技术。视实际应用需求而定。并在检测点收集SERS信号。Hg2 +的最低检出限为10-9M。应该指出的是，该研究不仅限于Hg2 +定量分析，而且主要目的是开发一种用于设计和制造新型自驱动微流控装置的新技术。根据实际应用要求。