Organic fluorescent bioprobes provide robust and powerful analytical techniques for direct detection and monitoring of important biological species and their related biological processes in live samples. In contrast to conventional fluorophores with aggregation-caused quenching effect, aggregation-induced emission (AIE) luminogens (AIEgens) exhibit remarkable photophysical properties such as bright fluorescence in aggregate or solid state, large Stokes shift, excellent photostability, and long retention time in live samples. Benefiting from these advantages, AIEgen-based bioprobes have been widely applied in diverse biomedical applications. In this review, we first introduce the AIE concept and working principles of AIE bioprobes. Then we briefly summarize their applications in biosensing and bioimaging with some representative examples recently reported mainly in our group. The challenge and future development of AIE bioprobes are also discussed and highlighted. Hopefully, this review can inspire more researchers to participate in this fascinating area and expand the versatile biomedical applications.

有机荧光生物探针提供了强大而强大的分析技术，可直接检测和监测活体样品中的重要生物物种及其相关生物过程。与具有聚集引起猝灭作用的常规荧光团相比，聚集诱导发射（AIE）发光原（AIEgens）具有非凡的光物理特性，例如聚集或固态的明亮荧光，大的斯托克斯位移，出色的光稳定性和在活体内的长时间保留样品。受益于这些优势，基于AIEgen的生物探针已广泛应用于各种生物医学应用中。在本文中，我们首先介绍了AIE生物探针的AIE概念和工作原理。然后，我们简要总结它们在生物传感和生物成像中的应用，并列举一些最近在我们小组中主要报道的代表性实例。还讨论并强调了AIE生物探针的挑战和未来发展。希望这篇评论可以激发更多的研究人员参与这个引人入胜的领域，并扩展多功能生物医学的应用。