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Potential and limits of a colloid approach to protein solutions.
Soft Matter ( IF 3.4 ) Pub Date : 2020-01-02 , DOI: 10.1039/c9sm01953g
Anna Stradner 1 , Peter Schurtenberger
Affiliation  

Looking at globular proteins with the eyes of a colloid scientist has a long tradition, in fact a significant part of the early colloid literature was focused on protein solutions. However, it has also been recognized that proteins are much more complex than the typical hard sphere-like synthetic model colloids. Proteins are not perfect spheres, their interaction potentials are in general not isotropic, and using theories developed for such particles are thus clearly inadequate in many cases. In this perspective article, we now take a closer look at the field. In particular, we reflect on the fact that modern colloid science has been undergoing a tremendous development, where a multitude of novel systems have been developed in the lab and in silico. During the last decade we have seen a rapidly increasing number of reports on the synthesis of anisotropic, patchy and/or responsive synthetic colloids, that start to resemble their complex biological counterparts. This experimental development is also reflected in a corresponding theoretical and simulation effort. The experimental and theoretical toolbox of colloid science has thus rapidly expanded, and there is obviously an enormous potential for an application of these new concepts to protein solutions, which has already been realized and harvested in recent years. In this perspective article we make an attempt to critically discuss the exploitation of colloid science concepts to better understand protein solutions. We not only consider classical applications such as the attempt to understand and predict solution stability and phase behaviour, but also discuss new challenges related to the dynamics, flow behaviour and liquid-solid transitions found in concentrated or crowded protein solutions. It not only aims to provide an overview on the progress in experimental and theoretical (bio)colloid science, but also discusses current shortcomings in our ability to correctly reproduce and predict the structural and dynamic properties of protein solutions based on such a colloid approach.

中文翻译:

胶体方法对蛋白质溶液的潜力和局限性。

用胶体科学家的眼光看球状蛋白质已有很长的历史,实际上,早期胶体文献的很大一部分都集中在蛋白质溶液上。然而,也已经认识到蛋白质比典型的硬球状合成模型胶体复杂得多。蛋白质不是完美的球体,它们的相互作用潜能通常不是各向同性的,因此在许多情况下使用针对此类粒子开发的理论显然是不够的。在这篇有远见的文章中,我们现在将更仔细地研究该领域。特别是,我们反思了一个事实,即现代胶体科学正在经历巨大的发展,在实验室和计算机上已经开发了许多新颖的系统。在过去的十年中,我们已经看到关于各向异性,斑块状和/或响应性合成胶体的合成的报告迅速增加,这些胶体开始类似于它们的复杂生物学对应物。相应的理论和仿真工作也反映了这种实验性发展。因此,胶体科学的实验和理论工具箱已迅速扩展,显然,将这​​些新概念应用到蛋白质溶液中具有巨大的潜力,近年来已经实现并收获了这些新概念。在这篇观点文章中,我们尝试批判性地讨论对胶体科学概念的利用,以更好地理解蛋白质溶液。我们不仅考虑经典应用,例如试图理解和预测溶液稳定性和相行为,还讨论了与在浓缩或拥挤的蛋白质溶液中发现的动力学,流动行为和液固转变有关的新挑战。它不仅旨在概述实验和理论(生物)胶体科学的进展,而且还讨论了基于这种胶体方法正确再现和预测蛋白质溶液的结构和动态特性的能力的当前缺陷。
更新日期:2020-01-02
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