当前位置: X-MOL 学术Chem. Geol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of microbial growth rate on temperature and metabolic water recorded in 18O/16O ratios of PO4 in DNA
Chemical Geology ( IF 3.9 ) Pub Date : 2020-02-01 , DOI: 10.1016/j.chemgeo.2019.119439
Fei Wang , Chan Yu , Hui Li , Sae Jung Chang , Ruth E. Blake

Abstract It has been recently demonstrated that both temperature and intracellular/metabolic water are recorded by the PO4 moieties comprising the backbone of DNA in microbial cells as well as in total microbial biomass PO4 (Blake et al., 2016; Li et al., 2016). Temperature and intracellular water composition are reflected in the 18O/16O ratio of PO4 (δ18OP) in DNA (Blake et al., 2016). To determine whether the reported temperature recording by microbial DNA-PO4 could be an artifact of variable microbial growth rate, which may also vary as a function of temperature, three strains of microorganisms having distinct and different growth rate patterns between 23 and 42 °C—Pseudomonas fluorescens, Acinetobacter ADP1 and Marinobacter aquaeolei were cultured over a range of temperatures and growth rate patterns. Growth curve patterns for the different strains were distinct and growth rates increased with temperature. However, as we show here, variations in δ18OP values of DNA-PO4 did not correlate with changes in bacterial growth rates at a given temperature, and O isotope fractionations (i.e., partitioning of O isotopes) between PO4 in DNA and O in water were not significantly different between strains (

中文翻译:

DNA 中 PO4 的 18O/16O 比率记录的微生物生长速率对温度和代谢水的影响

摘要 最近已经证明,温度和细胞内/代谢水都由构成微生物细胞中 DNA 骨架的 PO4 部分以及总微生物生物质 PO4 记录(Blake 等人,2016 年;Li 等人,2016 年) )。温度和细胞内水成分反映在 DNA 中 PO4 (δ18OP) 的 18O/16O 比率中(Blake 等人,2016 年)。为确定微生物 DNA-PO4 报告的温度记录是否可能是微生物生长速率可变的假象,微生物生长速率也可能随温度而变化,三种微生物菌株在 23 至 42°C 之间具有不同的生长速率模式—— Pseudomonas fluorescens、Acinetobacter ADP1 和 Marinobacter aquaeoolei 在一系列温度和生长速率模式下培养。不同菌株的生长曲线模式不同,生长速率随温度升高而增加。然而,正如我们在这里展示的,DNA-PO4 的 δ18OP 值的变化与给定温度下细菌生长速率的变化无关,DNA 中的 PO4 和水中的 O 之间的 O 同位素分馏(即 O 同位素的分配)是菌株之间没有显着差异(
更新日期:2020-02-01
down
wechat
bug