Mesenchymal stem cells (MSCs) have unique potentials, including migration and immunomodulation. Identification of the factors that enhance these activities can improve clinical applications of MSCs. This study aimed to investigate total antioxidant capacity (TAC) and migration potential of mouse MSCs exposed to thymoquinone (TQ) in vitro, and to examine the effect of TQ-treated MSCs on the expression of mouse immune cell markers. The results of total antioxidant capacity and wound healing assays showed that TQ increased the rate of MSCs TAC and migration in a dose- and time-dependent manner. The maximum TAC and migration were detected at 600 and 250 ng/ml of TQ, respectively. Functionally, the real-time PCR data analysis indicated that TQ induced c-Met and Cxcr4 expression and therefore, there may be a correlation between upregulation of these genes and increased MSCs migration. TQ also enhanced the up and down regulating impact of MSCs on Rorγt and Plzf expression and the expression of Tcf4 in mouse immune cells, respectively. Overall, this study declares that TQ increases the TAC of MSCs. It also proposes that TQ may, through activation of c-MET and CXCR4 signalling pathways, promote MSCs migration. TQ may also augment MSCs immunogenicity through its influence on the expression of genes involved in commitment of mouse immune system cells in vivo.

中文翻译：

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胸腺醌在体外促进小鼠间充质干细胞迁移，并在体内诱导其免疫原性。

间充质干细胞（MSC）具有独特的潜力，包括迁移和免疫调节。确定增强这些活性的因素可以改善MSC的临床应用。这项研究旨在调查体外暴露于胸醌（TQ）的小鼠MSC的总抗氧化能力（TAC）和迁移潜力，并研究经TQ处理的MSC对小鼠免疫细胞标记物表达的影响。总抗氧化剂能力和伤口愈合试验的结果表明，TQ以剂量和时间依赖性方式增加了MSCs TAC和迁移的速率。在600和250 ng / ml的TQ下分别检测到最大TAC和迁移。从功能上讲，实时PCR数据分析表明TQ诱导了c-Met和Cxcr4表达，因此，这些基因的上调与MSCs迁移增加之间可能存在相关性。TQ还分别增强了MSCs对小鼠免疫细胞中Rorγt和Plzf表达以及Tcf4表达的上调和下调作用。总的来说，这项研究表明TQ增加了MSC的TAC。它还提出，TQ可以通过激活c-MET和CXCR4信号通路来促进MSC的迁移。TQ还可以通过影响体内参与小鼠免疫系统细胞的基因表达来增强MSC的免疫原性。通过激活c-MET和CXCR4信号通路，促进MSCs迁移。TQ还可以通过影响体内参与小鼠免疫系统细胞的基因表达来增强MSC的免疫原性。通过激活c-MET和CXCR4信号通路，促进MSCs迁移。TQ还可以通过影响体内参与小鼠免疫系统细胞的基因表达来增强MSC的免疫原性。