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Anti-cancer potential of polysaccharide extracted from hawthorn (Crataegus.) on human colon cancer cell line HCT116 via cell cycle arrest and apoptosis
Journal of Functional Foods ( IF 5.6 ) Pub Date : 2019-12-05 , DOI: 10.1016/j.jff.2019.103677
Lei Ma , Guanying Bianca Xu , Xinyu Tang , Cheng Zhang , Wen Zhao , Jie Wang , Hong Chen

This study demonstrated that a homogeneous polysaccharide (HPS) extracted from Hawthorn exerted anti-cancer effects on colon cancer cells. Human colon cancer cell line HCT116 were treated with 125–1000 µg/mL HPS for 12 h and subsequent analysis was performed on proliferation and signaling pathways. HPS significantly inhibited proliferation of HCT116 cells when analyzed using WST-1 assay and immunofluorescence staining of Ki-67. Flow cytometric studies revealed that HPS could arrest the cell cycle in the S and G2/M phases and increase the rate of apoptosis. HPS downregulated the expression of Cyclin A1/D1/E1 and CDK-1/2. HPS induced apoptosis on HCT116 cells through upregulation of caspase3, 7, 8, 9 and Fas, FADD, TNF-R1, TRADD, upregulation of caspase3 protein was also observed. HPS has an anti-cancer potential in the treatment of human colon cancer which makes it a potential candidate in functional foods for cancer patients.



中文翻译:

山楂(Crataegus。)多糖通过细胞周期阻滞和凋亡对人结肠癌细胞系HCT116的抗癌潜力

这项研究表明,从山楂提取的均质多糖(HPS)对结肠癌细胞具有抗癌作用。将人结肠癌细胞系HCT116用125–1000 µg / mL HPS处理12 h,然后对增殖和信号通路进行后续分析。使用WST-1分析和Ki-67免疫荧光染色分析时,HPS显着抑制HCT116细胞的增殖。流式细胞术研究表明,HPS可以阻止S和G2 / M期的细胞周期,并增加细胞凋亡率。HPS下调了Cyclin A1 / D1 / E1和CDK-1 / 2的表达。HPS通过上调caspase3、7、8、9和Fas,FADD,TNF-R1,TRADD诱导HCT116细胞凋亡,还观察到caspase3蛋白的上调。

更新日期:2019-12-05
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