Eight novel and three known antioxidant peptides isolated from protein hydrolysate of Moringa oleifera seeds, using ultrafiltration, anion-exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography (RP-HPLC). The novel peptides were identified as Gly-Tyr (GY), Pro-Phe-Glu (PFE), Tyr-Thr-Arg (YTR), Phe-Gly (FG), Gln-Tyr (QY), Ile-Asn (IN), Ser-Phe (SF), Ser-Pro (SP), and the known peptides were identified as Tyr-Phe-Glu (YFE), Ile-Tyr (IY) and Leu-Tyr (LY), respectively, using protein amino acid sequence analyzer and electrospray ionization-mass spectrometry. All the eleven peptides exhibited strong scavenging activities on 2,2-Diphenyl-1picrylhydrazyl radical (DPPH) (EC₅₀ 2.28, 1.60, 1.77, 2.15, 0.97, 1.30, 0.75, 0.91, 1.21, 0.79, and 1.37 mg/L, respectively) and 2,2-azino-bis (3-ethylbenzothia zoline-6-sulfonic acid) diammonium salt radical (ABTS⁺) (EC₅₀ 1.03, 0.84, 0.95, 0.65, 0.37, 0.54, 0.33, 0.36, 0.67, 0.32 and 0.38 mg/mL, respectively). In addition, SF and QY showed protective effects on oxidative damage Chang liver cells induced by H₂O₂, and the contents of aspartate aminotransferase (ALT), alanine aminotransferase (AST) and malondialdehyde (MDA) decreased with the increasing concentrations of SF and QY. Furthermore, SF and QY could significantly increase the levels of superoxide dismutase (SOD) and catalase (CAT), thereby effectively scavenge reactive oxygen species (ROS) in H₂O₂-induced oxidative damaged Chang liver cells (P < 0.05). These results suggested that SF and QY had the ability to protect Chang liver cells from oxidative stress damage and might serve as potential antioxidants or oxidative damage cytoprotective agents used in pharmaceutical and health food industries.

使用超滤，阴离子交换色谱，凝胶过滤色谱和反相高效液相色谱（RP-HPLC）从辣木种子的蛋白水解物中分离出八种新颖和三种已知的抗氧化剂肽。新的肽被鉴定为Gly-Tyr（GY），Pro-Phe-Glu（PFE），Tyr-Thr-Arg（YTR），Phe-Gly（FG），Gln-Tyr（QY），Ile-Asn（IN ），Ser-Phe（SF），Ser-Pro（SP）和已知的肽分别使用蛋白鉴定为Tyr-Phe-Glu（YFE），Ile-Tyr（IY）和Leu-Tyr（LY）氨基酸序列分析仪和电喷雾电离质谱。所有11种肽均对2,2-二苯基1-甲基肼基自由基（DPPH ）具有较强的清除活性（EC ₅₀分别为2.28、1.60、1.77、2.15、0.97、1.30、0.75、0.91、1.21、0.79和1.37 mg / L）和2,2-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐自由基（ABTS ⁺）（分别为EC ₅₀ 1.03、0.84、0.95、0.65、0.37、0.54、0.33、0.36、0.67、0.32和0.38 mg / mL）。此外，SF和QY对H ₂ O ₂诱导的Chang肝细胞氧化损伤具有保护作用，并且随着SF和QY浓度的增加，天冬氨酸转氨酶（ALT），丙氨酸转氨酶（AST）和丙二醛（MDA）的含量降低。 QY。此外，SF和QY可以显着增加超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的水平，从而有效清除H _2中的活性氧（ROS）O ₂诱导的氧化损伤的Chang肝细胞（P  <0.05）。这些结果表明，SF和QY具有保护Chang肝细胞免受氧化应激损伤的能力，并可能用作制药和保健食品行业中潜在的抗氧化剂或氧化损伤细胞保护剂。