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Comparison of oxidative stress induced by clarithromycin in two freshwater microalgae Raphidocelis subcapitata and Chlorella vulgaris.
Aquatic Toxicology ( IF 4.5 ) Pub Date : 2019-12-05 , DOI: 10.1016/j.aquatox.2019.105376
Jiahua Guo 1 , Jianglin Peng 1 , Yuan Lei 1 , Mirella Kanerva 2 , Qi Li 1 , Jinxi Song 1 , Jipu Guo 3 , Haotian Sun 1
Affiliation  

Clarithromycin (CLA), a macrolide antibiotic, has been frequently detected in the global surface waters. Concerns have been raised over the potential impacts of CLA on the non-target aquatic species, particularly algae acting as the primary producers in the ecosystem. This study therefore evaluated the toxicological effects of CLA at a range of concentration levels (0, 5, 20, 40, 80 μg L-1) on two green algae, Raphidocelis subcapitata (R. subcapitata) and Chlorella vulgaris (C. vulgaris). The algal growth, photosynthetic pigment contents, lipid peroxidation biomarker malondialdehyde (MDA), responses of antioxidants including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GP), and glutathione S-transferase (GST) were measured. After 7 d exposure, the growth of R. subcapitata was inhibited with the CLA exposure levels higher than 20 μg L-1, whereas the inhibition in C. vulgaris was detected at the concentration level of 80 μg L-1. The MDA contents in both species were elevated. To cope with the increased levels of ROS, the activities of enzymatic antioxidants (SOD, CAT, GP, and GST) and the content of non-enzymatic antioxidant (GSH) in R. subcapitata were all enhanced. However, in C. vulgaris, enhancement was detected only in the activities of antioxidant enzymes (SOD, CAT, and GP). In addition, chlorophyll a, b, and carotenoid contents were all significantly increased in R. subcapitata but decreased in C. vulgaris. The results suggested that R. subcapitata is more sensitive to CLA exposure than C. vulgaris. This study provides insights into the CLA - oxidative stress process in two algae.

中文翻译:

比较克拉霉素在两种淡水微藻类小角藻和小球藻中诱导的氧化应激。

大环内酯类抗生素克拉霉素(CLA)在全球地表水中经常被发现。人们对CLA对非目标水生物种,特别是作为生态系统主要生产者的藻类的潜在影响提出了关切。因此,本研究评估了浓度范围(0、5、20、40、80μgL-1)下的CLA对两种绿藻,分别是短笛实蝇(Raphidocelis subcapitata)和寻常绿藻(Chlorella vulgaris)(C。vulgaris)的毒理作用。 。藻类生长,光合色素含量,脂质过氧化生物标志物丙二醛(MDA),抗氧化剂的响应,包括超氧化物歧化酶(SOD),过氧化氢酶(CAT),谷胱甘肽(GSH),谷胱甘肽过氧化物酶(GP)和谷胱甘肽S-转移酶(GST)被测量。暴露7 d后,R的生长。当CLA暴露水平高于20μgL-1时,亚次生抑制被抑制,而在浓度为80μgL-1的寻常果梭状芽胞杆菌中则被抑制。两个物种中的MDA含量均升高。为了应对ROS水平的提高,人头小球藻中的酶促抗氧化剂(SOD,CAT,GP和GST)的活性和非酶促抗氧化剂(GSH)的含量都得到了增强。但是,在寻常衣藻中,仅在抗氧化酶(SOD,CAT和GP)的活性中检测到增强。此外,次生红景天中叶绿素a,b和类胡萝卜素的含量均显着增加,而寻常果假丝酵母中的叶绿素a,b和类胡萝卜素含量均明显降低。结果表明,次生R. subcapitata对CLA的暴露比寻常的C. gar更加敏感。这项研究为CLA-两种藻类的氧化应激过程提供了见识。
更新日期:2019-12-05
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