Synucleinopathies [Parkinson's disease with or without dementia, dementia with Lewy bodies and multiple system atrophy] are neurodegenerative diseases that are defined by the presence of filamentous α-synuclein inclusions. We investigated the ability of luminescent conjugated oligothiophenes to stain the inclusions of Parkinson's disease and multiple system atrophy. They stained the Lewy pathology of Parkinson's disease and the glial cytoplasmic inclusions of multiple system atrophy. Spectral analysis of HS-68-stained inclusions showed a red shift in multiple system atrophy, but the difference with Parkinson's disease was not significant. However, when inclusions were double-labelled for HS-68 and an antibody specific for α-synuclein phosphorylated at S129, they could be distinguished based on colour shifts with blue designated for Parkinson's disease and red for multiple system atrophy. The inclusions of Parkinson's disease and multiple system atrophy could also be distinguished using fluorescence lifetime imaging. These findings are consistent with the presence of distinct conformers of assembled α-synuclein in Parkinson's disease and multiple system atrophy.

中文翻译：

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发光共轭寡聚噻吩区分帕金森氏病的α-突触核蛋白装配和多系统萎缩。

突触核蛋白病[伴或不伴痴呆的帕金森氏病，路易体痴呆和多系统萎缩]是神经退行性疾病，其定义是存在丝状α-突触核蛋白包涵体。我们调查了发光共轭寡聚噻吩染色帕金森氏病和多系统萎缩的内含物的能力。他们对帕金森氏病的路易病理学和多系统萎缩的神经胶质细胞质内含物进行了染色。HS-68染色的夹杂物的光谱分析显示多系统萎缩呈红移，但与帕金森氏病的差异不显着。但是，当夹杂物被HS-68双重标记和对α-突触核蛋白特异的抗体在S129处磷酸化时，可以根据颜色变化来区分它们，蓝色表示帕金森氏病，红色表示多系统萎缩。帕金森氏病和多系统萎缩的包裹体也可以使用荧光寿命成像来区分。这些发现与帕金森氏病和多系统萎缩中组装的α-突触核蛋白不同构象体的存在一致。