A facile one-pot synthesis method for polyethyleneimine-functionalized graphene (PEI-G) was created by employing polyethyleneimine (PEI) as both the reductive and functionalized reagent to reduce and wrap exfoliated graphene oxide (EGO) to maintain the single layer status of graphene without aggregation. Based on the fine conductivity of PEI-G and high selectivity of the aptamer specially designed for kanamycin, a novel electrochemical impedance aptasensor for the highly-sensitive and selective detection of kanamycin in serum was fabricated using PEI-G as the electrode substrate to enhance the electrochemical signal and using the aptamer as a biological recognition unit to promote the selectivity. After the specific capture of kanamycin in the sample, the proposed aptasensor exhibited a fine line response for kanamycin within the range of 10 fM to 100 pM with a detection limit of 5.2 fM. Moreover, the proposed aptasensor also displayed a good reproducibility, stability and specificity, which was suitable for the trace kanamycin analyses in a real serum sample. Therefore, this proposed electrochemical impedance aptasensing strategy opens a new insight into potential applications in the clinical diagnostic use of kanamycin.

中文翻译：

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一种简便的一锅法合成聚乙烯亚胺官能化的石墨烯，可对血清中的卡那霉素进行高灵敏度和选择性的电化学阻抗适应性†

通过使用聚乙烯亚胺（PEI）作为还原剂和功能化试剂来还原和包裹脱落的氧化石墨烯（EGO）以维持石墨烯的单层状态，创建了一种简便的一锅合成聚乙烯亚胺官能化的石墨烯（PEI-G）的方法。没有聚集。基于PEI-G的优良电导率和专为卡那霉素设计的适体的高选择性，以PEI-G为电极底物，制备了一种用于血清中卡那霉素的高灵敏和选择性检测的新型电化学阻抗适体传感器，以增强电化学信号并使用适体作为生物识别单元来促进选择性。在样品中特异性捕获卡那霉素后，拟议的适体传感器在10 fM至100 pM的范围内对卡那霉素表现出细线响应，检出限为5.2 fM。此外，拟议的适体传感器还显示出良好的重现性，稳定性和特异性，适用于实际血清样品中的痕量卡那霉素分析。因此，这种拟议的电化学阻抗适体策略为卡那霉素在临床诊断中的潜在应用开辟了新的见解。