We aimed to capture the outstanding mechanical properties of meshes, manufactured using textile technologies, in thin biodegradable biphasic tissue-engineered scaffolds through encapsulation of meshes into porous structures formed from the same polymer. Our novel manufacturing process used thermally induced phase separation (TIPS), with ethylene carbonate (EC) as the solvent, to encapsulate a poly(lactic-co-glycolic acid) (PLGA) mesh into a porous PLGA network. Biphasic scaffolds (1 cm × 4 cm × 300 μm) were manufactured by immersing strips of PLGA mesh in 40 °C solutions containing 5% PLGA in EC, supercooling at 4 °C for 4 min, triggering TIPS by manually agitating the supercooled solution, and lastly eluting EC into 4 °C Milli-Q water. EC processing was rapid and did not compromise mesh tensile properties. Biphasic scaffolds exhibited a tensile strength of 40.7 ± 2.2 MPa, porosity of 94%, pore size of 16.85 ± 3.78 μm, supported HaCaT cell proliferation, and degraded in vitro linearly over the first ∼3 weeks followed by rapid degradation over the following three weeks. The successful integration of textile-type meshes yielded scaffolds with exceptional mechanical properties. This thin, porous, high-strength scaffold is potentially suitable for use in dermal wound repair or repair of tubular organs.

中文翻译：

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将超强聚乳酸-乙醇酸共聚物（PLGA）编织网集成到热诱导相分离（TIPS）PLGA多孔结构中，以产生适用于皮肤组织工程的薄型双相支架。

我们的目标是通过将网格封装到由相同聚合物形成的多孔结构中，以捕获在薄的可生物降解的双相组织工程支架中使用纺织技术制造的网格的出色机械性能。我们新颖的制造工艺使用热诱导相分离（TIPS），以碳酸亚乙酯（EC）为溶剂，将聚乳酸-乙醇酸（PLGA）筛网封装到多孔PLGA网络中。通过将PLGA网格条浸入EC中含有5％PLGA的40°C溶液中，在4°C下过冷4分钟，通过手动搅拌过冷溶液触发TIPS来制造双相支架（1 cm×4 cm×300μm）最后将EC洗脱到4°C Milli-Q水中。EC处理速度很快，并且不会影响网眼的拉伸性能。双相支架表现出40.7±2.2 MPa的拉伸强度，94％的孔隙率，16.85±3.78μm的孔径，支持HaCaT细胞增殖，并在最初的〜3周内在体外线性降解，随后在接下来的3周内迅速降解。纺织品型网的成功集成产生了具有卓越机械性能的脚手架。这种薄的，多孔的，高强度的支架潜在地适合用于真皮伤口修复或肾小管器官的修复。