A one-pot enzymatic assisted supercritical carbon dioxide (SC-CO₂) extraction was used to obtain isorhamnetin conjugates from Opuntia ficus-indica (L.) Mill (nopal). Box-Benhken design, discriminate analysis and principal component analysis were used to studt the influence of pressure, temperature, aqueous ethanol solution and time on the total extraction yield and the isorhamnetin conjugates recovery using the enzymes Rapidase Maxi Fruit (Rapidase) and Viscozyme. Isorhamnetin tri- and diglycoside were found in the extracts along with other phenolic compounds such as 3-O-methyl quercetin, 3-O-methyl kaempferol and luteolin. The isorhamnetin and phenolic profile patterns were dependent on the conditions used. Cellular antioxidant activity was positively correlated to isorhamnetin-3-O-glucosyl-rhamnoside and luteolin content. The best results were obtained by using Rapidase, a currently used enzyme for the winemaking industry. The use of enzymes under SC-CO₂ conditions affected the release of isorhamnetin conjugate profiles which induced remarkable differences in the cellular antioxidant activity.

使用一锅酶促辅助超临界二氧化碳（SC-CO ₂）萃取从仙人掌仙人掌（L.）Mill（nopal）获得异鼠李素结合物。采用Box-Benhken设计，判别分析和主成分分析方法研究了压力，温度，乙醇水溶液和时间对总酶切得率的影响，并使用Rapidase Maxi Fruit（Rapidase）和Viscozyme酶回收了异鼠李素结合物。提取物中发现异鼠李素三糖苷和二糖苷以及其他酚类化合物，例如3- O-甲基槲皮素，3- O-甲基山ka酚和木犀草素。异鼠李素和酚的分布模式取决于所使用的条件。细胞抗氧化活性与异鼠李素-3- O-葡萄糖基鼠李糖苷和木犀草素含量呈正相关。通过使用Rapidase（目前用于酿酒行业的酶）可获得最佳结果。在SC-CO ₂条件下使用酶会影响异鼠李素结合物谱的释放，从而引起细胞抗氧化活性的显着差异。