Supercritical carbon dioxide (SC−CO₂) extraction of dry leaves of Piper hispidum was employed to optimize the selective extraction of cinnamoyl pyrrolidine amides. Taguchi L-9 method and 3² factorial experiments were used in the methodology optimization. The extraction yield (1.92 %, 353 K/ and 20 MPa) was improved significantly to 3.62 % with organic solvents addition (5 % of methanol in the extraction). The chemical composition investigation of supercritical extracts led to the isolation of four compounds, not initially identified by GC–MS. The compound 2 (Z,E) and 3 (E,Z) were identified as the major cinnamoyl pyrrolidine amides (up to 74.11 %). Antioxidant activity assay of the extract obtained at 333 K/ 20 MPa presented an IC₅₀ of 228 μg/mL and AAI of 0.087. The amide 2 and 5 % co-solvent extracts were also capable of inhibiting HL-60 cells grow up to 73 %. The newly developed methodology was suitable to obtain selectively metabolites with biological potential.

利用超临界二氧化碳（SC-CO ₂）提取Piper hispidum的干叶来优化肉桂酰基吡咯烷酰胺的选择性提取。在方法学优化中使用了田口L-9方法和3 ^2个阶乘实验。通过添加有机溶剂（萃取中含5％的甲醇），萃取收率（1.92％，353 K /和20 MPa）显着提高至3.62％。对超临界萃取物的化学成分研究导致了四种化合物的分离，这些化合物最初并未通过GC-MS鉴定。化合物2（Z，E）和3（E，Z）被确认为主要的肉桂酰基吡咯烷酰胺（最高74.11％）。在333 K / 20 MPa下获得的提取物的抗氧化活性测定表明，IC ₅₀为228μg/ mL，AAI为0.087。酰胺2和5％助溶剂提取物也能够抑制HL-60细胞生长至73％。新开发的方法学适合于获得具有生物潜力的选择性代谢物。