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Large scale production of lacto-N-biose I, a building block of type I human milk oligosaccharides, using sugar phosphorylases.
Bioscience, Biotechnology, and Biochemistry ( IF 1.6 ) Pub Date : 2019-09-28 , DOI: 10.1080/09168451.2019.1670047
Mamoru Nishimoto 1
Affiliation  

Human milk oligosaccharides (HMOs) have drawn attention for their contribution to the explosive bifidobacterial growth in the intestines of neonates. We found that bifidobacteria can efficiently metabolize lacto-N-biose I (LNB), the major building blocks of HMOs, and we have developed a method to synthesize LNB by applying this system. We produced LNB on a kilogram scale by the method. This proved that, among the enterobacteria, only bifidobacteria can assimilate LNB, and provided the data that supported the explosive growth of bifidobacteria in neonates. Furthermore, we were also able to reveal the structure of LNB crystal and the low stability for heating at neutral pH, which has not been clarified so far. In this paper, using bifidobacteria and LNB as examples, I describe the research on oligosaccharide synthesis that was conducted by utilizing a sugar metabolism.Abbreviations: LNB: lacto-N-biose I; GNB: galacto-N-biose; HMOs: human milk oligosaccharides; GLNBP: GNB/LNB phosphorylase; NahK: N-acetylhexosamine 1-kinase; GalT: UDP-glucose-hexose-1-phosphate uridylyltransferase; GalE: UDP-glucose 4-epimerase; SP: sucrose phosphorylase.

中文翻译:

使用糖磷酸化酶大规模生产乳酸-N-二糖I,这是I型人乳寡糖的组成部分。

人乳寡糖(HMO)由于其对新生儿肠道中爆炸性双歧杆菌生长的贡献而备受关注。我们发现双歧杆菌可以有效代谢HMO的主要组成部分-乳-N-二糖I(LNB),并且我们已经开发出一种通过应用该系统合成LNB的方法。通过这种方法,我们以千克规模生产了LNB。这证明,在肠杆菌中,只有双歧杆菌才能吸收LNB,并提供了支持双歧杆菌在新生儿中爆炸性增长的数据。此外,我们还能够揭示出LNB晶体的结构和在中性pH下加热的低稳定性,到目前为止尚未弄清。本文以双歧杆菌和LNB为例,我描述了利用糖代谢进行寡糖合成的研究。GNB:半乳糖-N-二糖;HMOs:人乳寡糖;GLNBP:GNB / LNB磷酸化酶;NahK:N-乙酰己糖胺1-激酶;GalT:UDP-葡萄糖-己糖-1-磷酸尿酸转移酶;GalE:UDP-葡萄糖4-表异构酶;SP:蔗糖磷酸化酶。
更新日期:2019-12-04
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