The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two‐photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism.

中文翻译：

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检测胎儿和成年绵羊脂肪和骨骼肌组织中的代谢差异。

产生ATP所需的主要代谢途径因组织类型，发育阶段和底物利用率而异。我们利用分子和组织学技术来定义胎儿和成人，脂肪和骨骼肌组织中的代谢状态。这些组织的氧化还原比也通过双光子显微镜光学确定。成人肾周脂肪组织比胎儿肾周脂肪组织具有更高的光学氧化还原比，这与糖酵解被用于生产ATP有关。而成年骨骼肌的光学氧化还原比要比胎儿骨骼肌低，这与需要ATP的需要氧化磷酸化活性的氧气相一致。